Multi-drug resistant bacteria in agricultural soil

• Published by: Environmental Information Data Centre
• Last updated: 26 September 2018
• Topic: Environment
• Licence: Other Licence ; View licence information

Summary

This dataset identified bacteria able to grow in the presence of several Antibiotics in a British agricultural soil, by DNA stable isotope probing (SIP). The dataset was created with samples of the 'heavy' and 'light' fractions of the treatments and also from control soils. The 16S rRNA genes from these samples were amplified and sequenced by barcoded Illumina sequencing. Polymerase chain reaction (PCR) primers 515FB (GTGYCAGCMGCCGCGGTAA) and 806RB (GGACTACNVGGGTWTCTAAT) from the Earth Microbiome project targeting the V4 region of the 16S rRNA gene (approximately 250 nucleotides) were used. Library preparation and sequencing was performed at the National Oceanographic Centre (NOC) of the University of Southampton, UK, following methodologies described by Caporaso et al. (2012). Samples were pooled in an equimolar concentration and sequenced on separate runs for MiSeq using a 2 bp x 300 bp paired end protocol.

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Link to the data	Format	File added	Data preview
Access the data	N/A	26 September 2018	Not available

Additional information

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• Added to data.gov.uk: 2018-09-11
• Access contraints: Not specified
• Harvest GUID: 595bca1f-9299-45d0-a224-a28ed136ea91
• Extent: Latitude: 50.97° to 50.96°
• Extent: Longitude: -1.43° to -1.42°
• Dataset reference date: 2018-01-05 (publication)
• Frequency of update: notPlanned
• Responsible party: University of Southampton (pointOfContact); EIDC (mediator)
• ISO 19139 resource type: dataset
• Metadata language: eng
• Source Metadata: XML
• Source Metadata: HTML

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