Identification

Title

Operational taxonomic units for epilithon bacteria in the rivers Wolf and Tamar catchments

Abstract

This dataset contains operational taxonomic units for epilithon (water samples): Approximate location of sampling sites was determined from maps to provide good spatial coverage of the Wold River through to the Tamar River. Exact sites were determined in the field, considering accessibility and other logistics. The exact location of each sample site was determined using a Garmin GPS12. Three stones were taken from each of the 20 locations and epilithon removed from a defined area. Samples were kept in the cold and removed to the laboratory for analyses. DNA was extracted from all soil and epilithon samples using the MOBIO Powersoil 96 well DNA extraction kit. DNA was quality checked for purity and yield prior to submission for 454 pyrosequencing to assess both bacterial and eukaryotic biodiversity within each sample. Following bioinformatic sequence processing, sequencing were clustered into operational taxonomic units (OTU) and the data tables display the percentage of each OTU within each sample. Full details about this dataset can be found at https://doi.org/10.5285/16649ff0-af24-41b0-bcb4-15e610dac170

Resource type

dataset

Resource locator

https://data-package.ceh.ac.uk/data/16649ff0-af24-41b0-bcb4-15e610dac170

name: Download the data

description: Get a copy of this data

function: download

https://data-package.ceh.ac.uk/sd/16649ff0-af24-41b0-bcb4-15e610dac170.zip

name: Supporting Information

description: Supporting information available to assist in re-use of this dataset

function: information

Unique resource identifier

code

1395928899368

codeSpace

CEH:EIDC:

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

biota

environment

inlandWaters

location

Keywords

Keyword set

keyword value

Land Use

originating controlled vocabulary

title

GEMET - INSPIRE themes, version 1.0

reference date

date type

publication

effective date

2008-06-01

Keyword set

keyword value

Soil

Environmental monitoring facilities

Species distribution

Hydrography

microbiology

genetic information

Tellus

South West project

Geographic location

West bounding longitude

-6.452

East bounding longitude

1.768

North bounding latitude

55.812

South bounding latitude

49.864

Temporal reference

Temporal extent

Begin position

2013-10-01

End position

2014-03-20

Dataset reference date

date type

publication

effective date

2014-05-14

date type

creation

effective date

2010-01-01

Frequency of update

unknown

Quality and validity

Lineage

Epilithon (water samples): Approximate location of sampling sites was determined from maps to provide good spatial coverage of the Wold River through to the Tamar River. Exact sites were determined in the field, considering accessibility and other logistics. The exact location of each sample site was determined using a Garmin GPS12. Three stones were taken from each of the 20 locations and epilithon removed from a defined area. Samples were kept in the cold and removed to the laboratory for analyses. DNA was extracted from all soil and epilithon samples using the MOBIO Powersoil 96 well DNA extraction kit. DNA was quality checked for purity and yield prior to submission for 454 pyrosequencing to assess both bacterial and eukaryotic biodiversity within each sample. Following bioinformatic sequence processing, sequencing were clustered into operational taxonomic units (OTU) and the data tables display the percentage of each OTU within each sample.

Conformity

Conformity report

specification

title

Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services

reference date

date type

publication

effective date

2010-12-08

degree

explanation

Data format

name of format

Comma-separated values (CSV)

version of format

Constraints related to access and use

Constraint set

Limitations on public access

Constraint set

Limitations on public access

Constraint set

Limitations on public access

Constraint set

Limitations on public access

If you reuse this data, you should cite: Griffiths, R., Edwards, F., Scarlett, P., Webb, G., Sarlej, R., Thomson, B. (2014). Operational taxonomic units for epilithon bacteria in the rivers Wolf and Tamar catchments. NERC Environmental Information Data Centre https://doi.org/10.5285/16649ff0-af24-41b0-bcb4-15e610dac170

owner

Metadata on metadata

Metadata point of contact

organisation name

Environmental Information Data Centre

full postal address

Lancaster Environment Centre, Library Avenue, Bailrigg

Lancaster

LA1 4AP

email address

info@eidc.ac.uk

responsible party role

pointOfContact

Metadata date

2021-06-25T18:44:50

Metadata language

eng