Global river cellulose decomposition assay data 2016-17
This dataset contains information regarding mountain river sites sampled in 2016-17. It includes: 1) physicochemical parameters of the river sites sampled, 2) tensile-strength loss data of a standardised cotton strip assay as a proxy for the decomposition of cellulose in mountain rivers, 3) molecular next generation sequencing data of the fungal Internal Transcribed Spacer region (ITS) and Cellobiohydrolase I gene (cbhI) sampled from the cotton strip decomposition assay. Field observations were made in Alaska, Austria, Ecuador, France, New Zealand and Norway to investigate how decreasing catchment glacier cover influences the fungal decomposition of organic matter in mountain rivers. Full details about this dataset can be found at https://doi.org/10.5285/fec704d2-ee6a-427b-9345-850dd96ff1b4
dataset
https://data-package.ceh.ac.uk/data/fec704d2-ee6a-427b-9345-850dd96ff1b4
name: Download the data
description: Download a copy of this data
function: download
https://data-package.ceh.ac.uk/sd/fec704d2-ee6a-427b-9345-850dd96ff1b4.zip
name: Supporting information
description: Supporting information available to assist in re-use of this dataset
function: information
https://catalogue.ceh.ac.uk/id/fec704d2-ee6a-427b-9345-850dd96ff1b4
doi:
eng
biota
inlandWaters
Environmental Monitoring Facilities
publication
2008-06-01
Next generation sequencing
qPCR
Cellobiohydrolase I (cbhI)
Cotton-strip assay
Mountain rivers
Decomposition assay
Tensile-strength data
NGS data
Physicochemical data
-180
180
90
-90
2016-01-01
2017-12-31
publication
2021-01-27
In the field, physicochemical parameters were obtained using a combination of in-situ probe measurements and geomorphological assessment, alongside collection of river water for ex-situ determination of some variables. A standardised cellulose (cotton strip) decomposition assay was deployed at each river site. In the laboratory, a tensiometer (University of Leeds) was used to determine the tensile-strength loss for incubated and control cotton strips, as a proxy for the decomposition of cellulose. Tensile-strength loss values were standardised by days of incubation and water temperature. DNA was extracted from subsamples of the cotton strips and next generation sequencing (qPCR, PCR) (University of Essex) performed to estimate the abundance of fungi (ITS), subgroups of fungi (saprotrophs, Ascomycota, Tetracladium) and a fungal gene (cbhI). Molecular data was subjected to quality control prior to taxonomic classification and assignment of trophic mode.
publication
2010-12-08
Comma-separated values (CSV)
If you reuse this data, you should cite: Fell, S. , Carrivick, J. L., Cauvy-Fraunié, S., Crespo-Pérez, V., Hood, E., Randall, K. C., Matthews Nicholass, K. J., Tiegs, S. D., Dumbrell, A. J., Brown, L. E. (2021). Global river cellulose decomposition assay data 2016-17. NERC Environmental Information Data Centre https://doi.org/10.5285/fec704d2-ee6a-427b-9345-850dd96ff1b4
University of Leeds
author
University of Leeds
author
INRAE
Sophie.cauvy-fraunie@irstea.fr
author
Pontifical Catholic University of Ecuador
author
University of Alaska Southeast
author
University of Essex
author
University of Essex
author
Oakland University
author
University of Essex
author
University of Leeds
author
NERC EDS Environmental Information Data Centre
custodian
NERC Environmental Information Data Centre
publisher
University of Leeds
pointOfContact
Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg
Lancaster
LA1 4AP
UK
pointOfContact
2021-06-25T18:32:48