Identification

Title

Post-fire microbial data (16S DNA) from burned peat soil on the Stalybridge estate (UK), October 2018

Abstract

This dataset contains information on the Bacterial Amplicon Sequence Variant (ASV) abundance from peat soil sampled following a wildfire on the Stalybridge estate (UK) in June 2020. Samples were taken in 10 established plots at three time periods following the fire: approximately 3 months, 10 months and 12 months post-fire. This was taken at two depths: 0 – 5cm from the surface (top) and 5 – 10 cm from the surface (bottom). The 10 plots were divided into two groups of five, one that was observed to have suffered a light burn (termed shallow burn) and one that received a more severe burn (termed deep burn). Five additional plots were sampled at the same time as the 3-month samples from a neighbouring unburned site (termed control plots). Samples where no ASVs were observed were removed from the dataset. Full details about this dataset can be found at https://doi.org/10.5285/01ced3c2-17c6-4512-b73b-e065afed7bad

Resource type

dataset

Resource locator

https://data-package.ceh.ac.uk/data/01ced3c2-17c6-4512-b73b-e065afed7bad

name: Download the data

description: Download a copy of this data

function: download

https://data-package.ceh.ac.uk/sd/01ced3c2-17c6-4512-b73b-e065afed7bad.zip

name: Supporting information

description: Supporting information available to assist in re-use of this dataset

function: information

Unique resource identifier

code

https://catalogue.ceh.ac.uk/id/01ced3c2-17c6-4512-b73b-e065afed7bad

codeSpace

doi:

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

biota

environment

Keywords

Keyword set

keyword value

Environmental Monitoring Facilities

originating controlled vocabulary

title

GEMET - INSPIRE themes, version 1.0

reference date

date type

publication

effective date

2008-06-01

Keyword set

keyword value

Blanket bog

Moorland

Peatland

DNA

16S rRNA

Bacterial Amplicon Sequence Variant

Geographic location

West bounding longitude

-1.966

East bounding longitude

-1.942

North bounding latitude

53.546

South bounding latitude

53.542

Temporal reference

Temporal extent

Begin position

2018-10-01

End position

2018-10-31

Dataset reference date

date type

publication

effective date

2022-01-18

Frequency of update

Quality and validity

Lineage

DNA was extracted from the peat soil using the QIAGEN DNeasy powersoil kit, following standard manual specifications. The extracted DNA was then sequenced through the use of high-throughput sequencing, using 515F/806R primers that target the V4 region of the 16S rRNA. Results from the sequencing were then cleaned and prepared using the DADA2 pipeline to produce the ASV table.

Conformity

Conformity report

specification

title

Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services

owner

Metadata on metadata

Metadata point of contact

organisation name

Environmental Information Data Centre

full postal address

Lancaster Environment Centre, Library Avenue, Bailrigg

Lancaster

LA1 4AP

email address

info@eidc.ac.uk

responsible party role

pointOfContact

Metadata date

2022-08-30T12:19:08

Metadata language

eng