Identification

Title

Soil fungi gene copy numbers and community composition under birch and willow vegetation in girdled and non-girdled plots, subarctic Sweden, 2017-2019

Abstract

Fungal community DNA was sequenced from soils in northern, subarctic Sweden. Soils were sampled from treeline mountain birch forest and stands of tundra willow that were wither ‘girdled’ (connection between leaves and roots cut by disrupting the phloem transport) or ‘control’ (no treatment), 0, 1 and 2 years after the treatment was applied. Full details about this dataset can be found at https://doi.org/10.5285/d6c787ec-146c-461b-b8a2-e0251259036c

Resource type

dataset

Resource locator

https://data-package.ceh.ac.uk/data/d6c787ec-146c-461b-b8a2-e0251259036c

name: Download the data

description: Download a copy of this data

function: download

https://data-package.ceh.ac.uk/sd/d6c787ec-146c-461b-b8a2-e0251259036c.zip

name: Supporting information

description: Supporting information available to assist in re-use of this dataset

function: information

Unique resource identifier

code

https://catalogue.ceh.ac.uk/id/d6c787ec-146c-461b-b8a2-e0251259036c

codeSpace

doi:

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

biota

environment

Keywords

Keyword set

keyword value

Environmental Monitoring Facilities

originating controlled vocabulary

title

GEMET - INSPIRE themes, version 1.0

reference date

date type

publication

effective date

2008-06-01

Keyword set

keyword value

Subarctic

Treeline

Tundra

Soil funngi

Girdling

Geographic location

West bounding longitude

12.017

East bounding longitude

22.395

North bounding latitude

71.663

South bounding latitude

55.106

Temporal reference

Temporal extent

Begin position

2017-08-03

End position

2019-08-05

Dataset reference date

date type

publication

effective date

2022-08-31

Frequency of update

Quality and validity

Lineage

The organic soil horizon (O horizon) was sampled on 3rd August 2017, 1st August 2018 and 5th August 2019. For each plot (22 in total), nine 3.8 cm diameter soil cores were collected; in the birch plots, cores were taken in a grid across a 3 x 3 m central area (corresponding to the central area in which soil CO2 efflux, root and mycelium production and soil fauna measurements were taken; (Parker et al., 2020). In the willow plots, the nine cores were distributed evenly across the plot area within the trenched perimeter. The uppermost litter layer, as well as the mineral horizons underlying the organic horizon (clearly identifiable at these sites), were removed from cores immediately after coring. The nine cores from each plot were homogenised and pooled within 6 hours of sampling. DNA was extracted from a 50 mg sub-sample and ITS2 markers were amplified by PCR. We used a community metabarcoding approach on the pooled DNA samples to identify distinct taxonomic groups at species level where possible. Copy numbers of the fungal ITS2 region were estimated by quantitative PCR (qPCR)

Conformity

Conformity report

specification

title

Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services

reference date

date type

publication

effective date

2010-12-08

degree

explanation

Data format

name of format

Comma-separated values (CSV)

version of format

Constraints related to access and use

Constraint set

Limitations on public access

Constraint set

Limitations on public access

Constraint set

Limitations on public access

If you reuse this data, you should cite: Parker, T.C., Chomel, M., Clemmensen, K.E., Friggens, N.L., Hartley, I.P., Johnson, D., Krab, E.J., Lindahl, B.D., Street, L.E., Subke, J.-A., Wookey, P.A. (2022). Soil fungi gene copy numbers and community composition under birch and willow vegetation in girdled and non-girdled plots, subarctic Sweden, 2017-2019. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/d6c787ec-146c-461b-b8a2-e0251259036c

Responsible organisations

Responsible party

organisation name

The James Hutton Institute

Identification

Title

Abstract

Resource type

Resource locator

Unique resource identifier

code

codeSpace

Dataset language

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

Keywords

Keyword set

keyword value

originating controlled vocabulary

title

reference date

date type

effective date

Keyword set

keyword value

Geographic location

West bounding longitude

East bounding longitude

North bounding latitude

South bounding latitude

Temporal reference

Temporal extent

Begin position

End position

Dataset reference date

date type

effective date

Frequency of update

Quality and validity

Lineage

Conformity

Conformity report

specification

title

reference date

date type

effective date

degree

explanation

Data format

name of format

version of format

Constraints related to access and use

Constraint set

Limitations on public access

Constraint set

Limitations on public access

Constraint set

Limitations on public access

Responsible organisations

Responsible party

organisation name

email address

responsible party role

Responsible party

organisation name

email address

responsible party role

Responsible party

organisation name

email address

responsible party role

Responsible party

organisation name

email address

responsible party role

Responsible party

organisation name

email address

responsible party role

Responsible party

organisation name