Identification

Title

Microbial DNA extraction and PCR from core samples SSK111456 and SSK111457 from UKGEOS Glasgow Observatory borehole GGC01

Abstract

Loan IDA number IDA271576. Microbial DNA concentrations from DNA extraction using Qiagen PowerSoil Pro DNA Extraction kit and subsequent PCR from shale and sandstone core samples collected and preserved for microbiology (shale SSK111456 and sandstone SSK111457) from UKGEOS Glasgow Observatory, borehole GGC01. DNA extractions and subsequent amplification using PCR indicate levels of microbial DNA below detection limits. Samples and data are derived from the UK Geoenergy Observatories Programme funded by the UKRI Natural Environment Research Council and delivered by the British Geological Survey.

Resource type

dataset

Resource locator

https://webapps.bgs.ac.uk/services/ngdc/accessions/index.html#item166985

name: Data

function: download

Unique resource identifier

code

http://data.bgs.ac.uk/id/dataHolding/13607775

codeSpace

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

geoscientificInformation

Keywords

Keyword set

keyword value

originating controlled vocabulary

title

reference date

date type

publication

effective date

2008-06-01

Keyword set

keyword value

Geomicrobiology

Shale

Sandstone

originating controlled vocabulary

title

reference date

date type

revision

effective date

2011

Keyword set

keyword value

NERC_DDC

Geographic location

West bounding longitude

-4.2216

East bounding longitude

-4.2216

North bounding latitude

55.8411

South bounding latitude

55.8411

Extent

Extent group

authority code

title

reference date

date type

revision

effective date

2002

code identifying the extent

Glasgow [id=1298677]

Temporal reference

Temporal extent

Begin position

2020-03-01

End position

2020-08-31

Dataset reference date

date type

creation

effective date

2021-06-22

Frequency of update

notApplicable

Quality and validity

Lineage

Detailed methodology in attached documents. Core samples processed into powder in sterile conditions. Powdered core then used for DNA extractions in Qiagen PowerSoil Pro DNA Extraction kit. Resulting elutes amplified using Polymerase Chain Reaction (PCR). PCR products visualised using gel electrophoresis.

Conformity

Conformity report

specification

title

reference date

date type

publication

effective date

2011

degree

false

explanation

See the referenced specification

Conformity report

specification

title

reference date

date type

publication

effective date

2010-12-08

degree

false

explanation

See http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2010:323:0011:0102:EN:PDF

Data format

name of format

MS Excel

version of format

Constraints related to access and use

Constraint set

Limitations on public access

Constraint set

Limitations on public access

The copyright of materials derived from the British Geological Survey's work is vested in the Natural Environment Research Council [NERC]. No part of this work may be reproduced or transmitted in any form or by any means, or stored in a retrieval system of any nature, without the prior permission of the copyright holder, via the BGS Intellectual Property Rights Manager. Use by customers of information provided by the BGS, is at the customer's own risk. In view of the disparate sources of information at BGS's disposal, including such material donated to BGS, that BGS accepts in good faith as being accurate, the Natural Environment Research Council (NERC) gives no warranty, expressed or implied, as to the quality or accuracy of the information supplied, or to the information's suitability for any use. NERC/BGS accepts no liability whatever in respect of loss, damage, injury or other occurence however caused.

Responsible organisations

Responsible party

contact position

PhD Student School of Earth Sciences

organisation name

University of Bristol

full postal address

Wills Memorial Building, Queens Road, Clifton

Bristol

BS8 1RJ

email address

not available

responsible party role

originator

Responsible party

organisation name

British Geological Survey

email address

not available

responsible party role

distributor

Responsible party

organisation name

British Geological Survey

email address

not available

responsible party role

pointOfContact

Metadata on metadata

Metadata point of contact

organisation name

British Geological Survey

full postal address

Environmental Science Centre,Keyworth

NOTTINGHAM

NG12 5GG

United Kingdom

telephone number

+44 115 936 3100

email address

enquiries@bgs.ac.uk

responsible party role

pointOfContact

Metadata date

2022-05-18

Metadata language