27da4400-9835-4241-9df9-a3b598f8d274
English
ISO/IEC 8859-1 (also known as Latin 1)
dataset
dataset
Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg
Lancaster
LA1 4AP
UK
info@eidc.ac.uk
pointOfContact
2020-09-17T10:52:57
UK GEMINI
2.3
OSGB 1936 / British National Grid
Genotype data for three freshwater macroinvertebrate species in upland Wales, UK (2012-2013)
2017-09-20
publication
2013-06-15
creation
https://catalogue.ceh.ac.uk/id/27da4400-9835-4241-9df9-a3b598f8d274
10.5285/27da4400-9835-4241-9df9-a3b598f8d274
doi:
MacDonald, H.C., Bruford, M.W., Ormerod, S., Jones, T.H. (2017). Genotype data for three freshwater macroinvertebrate species in upland Wales, UK (2012-2013). NERC Environmental Information Data Centre 10.5285/27da4400-9835-4241-9df9-a3b598f8d274
The resource consists of genotype data (genetic constitution of an individual organism) for three freshwater macroinvertebrate species, Amphinemura sulcicollis, Isoperla grammatica and Baetis rhodani collected at sites in upland Wales during May 2012 and May 2013. New nucleotide tandem repeats in DNA sequences i.e. Novel microsatellites were developed for this project so that genetic analysis could be carried out within the three species. Extra data on how microsatellite scores were grouped i.e. binned into unique alleles is provided, to aid replication. The data was generated for Hannah Macdonald's PhD thesis: to study genetic structure and diversity, demographic species history and resilience in freshwater ecosystems.
The work was carried out under Diversity in Upland Rivers for Ecosystem Service Sustainability (DURESS) project (Grant reference NERC NE/J014818/1). DURESS was funded by the Natural Environment Research Council (NERC) under the Biodiversity and Ecosystem Service Sustainability (BESS) programme. Full details about this dataset can be found at https://doi.org/10.5285/27da4400-9835-4241-9df9-a3b598f8d274
Hannah MacDonald
Cardiff University
Hannah.c.burton@gmail.com
pointOfContact
MacDonald, H.C.
Cardiff University
Hannah.c.burton@gmail.com
author
Bruford, M.W.
Cardiff University
BrufordMW@cardiff.ac.uk
author
Ormerod, S.
Cardiff University
Ormerod@cardiff.ac.uk
author
Jones, T.H.
Cardiff University
JonesTH@cardiff.ac.uk
author
Environmental Information Data Centre
info@eidc.ac.uk
custodian
NERC Environmental Information Data Centre
info@eidc.ac.uk
publisher
Cardiff University
enquiries@ceh.ac.uk
owner
notPlanned
Habitats and Biotopes
theme
GEMET - INSPIRE themes, version 1.0
2008-06-01
publication
Amphinemura sulcicollis
Isoperla grammatica
Baetis rhodani
Wikidata
2012-10-29
creation
Diversity of Upland Rivers for Ecosystem Service Sustainability (DURESS)
Microsatellites
Fragment analysis
Genetic diversity
Freshwater invertebrates
otherRestrictions
no limitations
otherRestrictions
This resource is made available under the terms of the Open Government Licence
otherRestrictions
© Cardiff University
otherRestrictions
If you reuse this data, you should cite: MacDonald, H.C., Bruford, M.W., Ormerod, S., Jones, T.H. (2017). Genotype data for three freshwater macroinvertebrate species in upland Wales, UK (2012-2013). NERC Environmental Information Data Centre https://doi.org/10.5285/27da4400-9835-4241-9df9-a3b598f8d274
textTable
English
utf8
biota
2012-06-15
2013-06-15
-4.073
-3.08
51.921
53.123
Comma-separated values (CSV)
Environmental Information Data Centre
info@eidc.ac.uk
distributor
https://data-package.ceh.ac.uk/data/27da4400-9835-4241-9df9-a3b598f8d274
Download the data
Download a copy of this data
download
https://data-package.ceh.ac.uk/sd/27da4400-9835-4241-9df9-a3b598f8d274.zip
Supporting information
Supporting information available to assist in re-use of this dataset
information
dataset
dataset
Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
2010-12-08
Samples of each invertebrate were collected by kicksampling in May in 2012 and 2013, samples were sorted and identified to genus level on site and stored in absolute ethanol. In the laboratory each larva was identified to species level using Elliott et al. (1988) and Hynes (1977). DNA was then extracted from tissue samples (see supporting information for extraction methods). Baetis rhodani samples contained cryptic species which had to be removed before genotyping, no cryptic species were identified for Amphinemura sulcicollis or Isoperla grammatica. Samples were sent for fragment analysis (Dundee Biosciences, UK) using ROX500 as the internal size standard. Fragment analysis products were visualised and scored using Genemarker v 1.91 (Holland and Parson 2011). These results were downloaded from Genemarker and allele sizes were binned by hand using the 'Binning' information (provided in the metadata) in an Excel spreadsheet. The data were then complied into master Excel spreadsheets for each invertebrate. The data were then exported as comma separated value files for ingestion into the EIDC.