Genotype data for three freshwater macroinvertebrate species in upland Wales, UK (2012-2013)
The resource consists of genotype data (genetic constitution of an individual organism) for three freshwater macroinvertebrate species, Amphinemura sulcicollis, Isoperla grammatica and Baetis rhodani collected at sites in upland Wales during May 2012 and May 2013. New nucleotide tandem repeats in DNA sequences i.e. Novel microsatellites were developed for this project so that genetic analysis could be carried out within the three species. Extra data on how microsatellite scores were grouped i.e. binned into unique alleles is provided, to aid replication. The data was generated for Hannah Macdonald's PhD thesis: to study genetic structure and diversity, demographic species history and resilience in freshwater ecosystems. The work was carried out under Diversity in Upland Rivers for Ecosystem Service Sustainability (DURESS) project (Grant reference NERC NE/J014818/1). DURESS was funded by the Natural Environment Research Council (NERC) under the Biodiversity and Ecosystem Service Sustainability (BESS) programme. Full details about this dataset can be found at https://doi.org/10.5285/27da4400-9835-4241-9df9-a3b598f8d274
dataset
https://data-package.ceh.ac.uk/data/27da4400-9835-4241-9df9-a3b598f8d274
name: Download the data
description: Download a copy of this data
function: download
https://data-package.ceh.ac.uk/sd/27da4400-9835-4241-9df9-a3b598f8d274.zip
name: Supporting information
description: Supporting information available to assist in re-use of this dataset
function: information
https://catalogue.ceh.ac.uk/id/27da4400-9835-4241-9df9-a3b598f8d274
doi:
eng
biota
Habitats and Biotopes
publication
2008-06-01
creation
2012-10-29
Diversity of Upland Rivers for Ecosystem Service Sustainability (DURESS)
Microsatellites
Fragment analysis
Genetic diversity
Freshwater invertebrates
-4.073
-3.08
53.123
51.921
2012-06-15
2013-06-15
publication
2017-09-20
creation
2013-06-15
notPlanned
Samples of each invertebrate were collected by kicksampling in May in 2012 and 2013, samples were sorted and identified to genus level on site and stored in absolute ethanol. In the laboratory each larva was identified to species level using Elliott et al. (1988) and Hynes (1977). DNA was then extracted from tissue samples (see supporting information for extraction methods). Baetis rhodani samples contained cryptic species which had to be removed before genotyping, no cryptic species were identified for Amphinemura sulcicollis or Isoperla grammatica. Samples were sent for fragment analysis (Dundee Biosciences, UK) using ROX500 as the internal size standard. Fragment analysis products were visualised and scored using Genemarker v 1.91 (Holland and Parson 2011). These results were downloaded from Genemarker and allele sizes were binned by hand using the 'Binning' information (provided in the metadata) in an Excel spreadsheet. The data were then complied into master Excel spreadsheets for each invertebrate. The data were then exported as comma separated value files for ingestion into the EIDC.
publication
2010-12-08
Comma-separated values (CSV)
© Cardiff University
If you reuse this data, you should cite: MacDonald, H.C., Bruford, M.W., Ormerod, S., Jones, T.H. (2017). Genotype data for three freshwater macroinvertebrate species in upland Wales, UK (2012-2013). NERC Environmental Information Data Centre https://doi.org/10.5285/27da4400-9835-4241-9df9-a3b598f8d274
Cardiff University
pointOfContact
Cardiff University
author
Cardiff University
author
Cardiff University
author
Cardiff University
author
Environmental Information Data Centre
custodian
NERC Environmental Information Data Centre
publisher
Cardiff University
owner
Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg
Lancaster
LA1 4AP
UK
pointOfContact
2020-09-17T10:52:57