Identification

Title

Taxonomic data, neuropil volume measurements, and mushroom body calyx kenyon cell and synapse counts for Heliconiini butterflies, Central and South America, 2012-2016

Abstract

This dataset contains taxanomic and neuropil volume measurements of wild caught heliconiine butterflies, as well as kenyon cell and synapse counts in the mushroom body calyx of insectory -reared heliconiine butterflies. Wild caught butterflies were sampled from five locations in Central and South America between 2012 and 2016. Locations included Costa Rica, Panama, French Guiana, Ecuador, and Peru. These locations covered a range of habitats from dry to wet forest, at elevations from sea level to 2500m. Interspecific data from wild caught individuals include identification to a species level, sex, and body size, as well as volume measurements of the mushroom body structures, central brain, medulla, and antennal lobe. Measurements of mushroom body calyx volume, and estimates of Kenyon cell and synapse counts are included for six species of insectary-reared adult heliconiine butterflies (Agraulis vanilla, dryas iulia, eueides Isabella, heliconius melpmonene, heliconius Hortense, heliconius hecale). Two species (dryas iulia, heliconius charthonia) were also sampled for microglomeruli counts. This dataset was created as part of a NERC Independent Research Fellowship NE/N014936/1, to analyse the evolutionary expansions in mushroom body size in Heliconius butterflies, compared to their closest relatives. Full details about this dataset can be found at https://doi.org/10.5285/de62eff7-6ea4-44d7-a015-2eb9b25cf882

Resource type

dataset

Resource locator

https://data-package.ceh.ac.uk/data/de62eff7-6ea4-44d7-a015-2eb9b25cf882

description: Download a copy of this data

function: download

https://data-package.ceh.ac.uk/sd/de62eff7-6ea4-44d7-a015-2eb9b25cf882.zip

description: Supporting information available to assist in re-use of this dataset

function: information

Unique resource identifier

code

https://catalogue.ceh.ac.uk/id/de62eff7-6ea4-44d7-a015-2eb9b25cf882

codeSpace

doi:

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

biota

Keywords

Keyword set

keyword value

Species Distribution

originating controlled vocabulary

title

GEMET - INSPIRE themes, version 1.0

reference date

date type

publication

effective date

2008-06-01

Geographic location

West bounding longitude

-119.308

East bounding longitude

-33.879

North bounding latitude

33.669

South bounding latitude

-56.207

Temporal reference

Temporal extent

Begin position

2012-01-01

End position

2016-12-31

Dataset reference date

date type

publication

effective date

2024-11-15

Frequency of update

Quality and validity

Lineage

Samples were collected over multiple field seasons across central and south America using a hand-net, fixed in situ and returned to the UK. Fixed samples were then stained with Anti-SYNORF as a primary antibody, cy2 as a secondary antibody, and imaged using confocal microscopy. The resulting images, which highlight boundaries between brain regions, were then segmented using AMIRA software to quantify neuropil volumes. Insectary-reared samples were obtained using pupae from commercial supplies. Freshly eclosed butterflies were aged in a controlled temperature room at 28 degrees Celsius with 80% humidity with a 12-hour day/night cycle. The cage was enriched with a natural food resource, supplemented by artificial feeders. Butterflies were sampled six to ten days after emergence. Kenyon cell counts were estimated using immunohistochemical staining against DAPI, imaged with confocal microscopy. Synapse density estimates were made using higher magnification imaging of Anti-SYNORF stained brains, focusing on the calyx. Total count data was obtained by estimating nuclei density/synapse density within sub-sampled regions of a structure, and then extrapolating across the total volume of the structure.

Conformity

Conformity report

specification

title

Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services

reference date

date type

publication

effective date

2010-12-08

degree

explanation

Data format

name of format

Comma-separated values (CSV)

version of format

Constraints related to access and use

Constraint set

Limitations on public access

Constraint set

Limitations on public access

Constraint set

Limitations on public access

If you reuse this data, you should cite: Montgomery, S., Couto, A. (2024). Taxonomic data, neuropil volume measurements, and mushroom body calyx kenyon cell and synapse counts for Heliconiini butterflies, Central and South America, 2012-2016. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/de62eff7-6ea4-44d7-a015-2eb9b25cf882

Responsible organisations

Responsible party

organisation name

email address

s.montgomery@bristol.ac.uk

web address

https://orcid.org/0000-0002-5474-5695

description: ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers.

function: information