Identification

Title

Tsetse trypanosome and endosymbiont data from Hurungwe District, Zimbabwe (2016)

Abstract

The data set includes the results of a laboratory analysis in 2016, investigating the presence of trypanosomes and prevalence of tsetse endosymbionts in tsetse flies. The tsetse flies were sampled in Hurungwe District, Mashonaland West Province, Zimbabwe, from February 2014 to November 2014. Flies were sampled using a combination of Epsilon traps and fly rounds, both established techniques for sampling tsetse. Tsetse were stored prior to laboratory analysis using Polymerase chain reaction (PCR) techniques in 2016. The data include two species of tsetse, Glossina pallidipes and Glossina morsitans morsitans. Trypanosome species investigated include Trypanosoma brucei s.l., T. b. rhodesiense, T. vivax, T. congolense, T. simiae, T.simiae (Tsavo) and T. godfreyi. Endosymbionts included in the study were Sodalis glossinidius and Wolbachia spp. Hurungwe District is the only sleeping sickness focus in Zimbabwe and an increase in cases had been detected in years preceding this study. The objective of the study was to investigate the trypanosome species present in the tsetse population and their association with tsetse endosymbionts. This study was conducted as part of research into the relationship between trypanosomiasis, well-being and ecosystems by the Dynamic Drivers of Disease in Africa Consortium (DDDAC). The research was funded by NERC with support from the Ecosystem Services for Poverty Alleviation Programme (ESPA). Full details about this dataset can be found at https://doi.org/10.5285/1e435dd3-ed93-4961-a136-ffa477731e1c

Resource type

dataset

Resource locator

https://data-package.ceh.ac.uk/data/1e435dd3-ed93-4961-a136-ffa477731e1c

name: Download the data

description: Download a copy of this data

function: download

https://data-package.ceh.ac.uk/sd/1e435dd3-ed93-4961-a136-ffa477731e1c.zip

name: Supporting information

description: Supporting information available to assist in re-use of this dataset

function: information

Unique resource identifier

code

https://catalogue.ceh.ac.uk/id/1e435dd3-ed93-4961-a136-ffa477731e1c

codeSpace

doi:

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

biota

health

Keywords

Keyword set

keyword value

originating controlled vocabulary

title

Wikidata

reference date

date type

creation

effective date

2012-10-29

Keyword set

keyword value

originating controlled vocabulary

title

GeoNames

reference date

date type

creation

effective date

2006-01-01

Keyword set

keyword value

Trypanosoma godfreyi

Dynamic Drivers of Disease in Africa Consortium (DDDAC)

Sleeping sickness

Geographic location

West bounding longitude

28.855

East bounding longitude

30.009

North bounding latitude

-15.929

South bounding latitude

-16.701

Temporal reference

Temporal extent

Begin position

2014-02-01

End position

2016-04-12

Dataset reference date

date type

publication

effective date

2017-04-21

date type

creation

effective date

2016-04-19

Frequency of update

notPlanned

Quality and validity

Lineage

The data presents the molecular analysis of 101 Glossina morsitans morsitans and 108 Glossina pallidipes tsetse sampled in Hurungwe District, Mashonaland West Province, Zimbabwe. Tsetse were sampled using Epsilon traps and fly rounds as described by Shereni et al. (2016). Tsetse were stored in cryotubes with desiccant beads at room temperature. DNA was extracted from the tsetse using Qiagen DNeasy kits and then subjected to polymerase chain reactions (PCR) to detect the primary endosymbiont, Wigglesworthia glossindia. Samples from which no DNA was amplified were discounted from further molecular analysis. All remaining samples were subjected to PCR reactions to detect African trypanosomes and the secondary tsetse symbionts, Sodalis glossinidius and Wolbachia. All PCRs included a negative and a positive control. The data were collated in an Excel spreadsheet and exported as a .csv file for ingestion into the EIDC.

Conformity

Conformity report

specification

title

Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services

Â© Central Veterinary Research and Diagnostic Laboratory, Zimbabwe

Constraint set

Limitations on public access

Â© Department of Livestock and Veterinary Services, Zimbabwe

Constraint set

Limitations on public access

If you reuse this data, you should cite: Goodwin, M., Webber, F., Hamill, L.C., Anderson, N.E., Shereni, W., Nyakupinda, L., Gwenhure, L.F., Macleod, E. (2017). Tsetse trypanosome and endosymbiont data from Hurungwe District, Zimbabwe (2016). NERC Environmental Information Data Centre https://doi.org/10.5285/1e435dd3-ed93-4961-a136-ffa477731e1c

custodian

Metadata on metadata

Metadata point of contact

organisation name

Environmental Information Data Centre

full postal address

Lancaster Environment Centre, Library Avenue, Bailrigg

Lancaster

LA1 4AP

email address

info@eidc.ac.uk

responsible party role

pointOfContact

Metadata date

2020-03-13T14:24:11

Metadata language

eng