Identification

Title

Paternity of Eschscholzia californica plants introduced to habitats comprising different floral cover

Abstract

This dataset details the paternity of progeny from Eschscholzia californica plants introduced to habitats comprising different floral cover. Data was collected in June 2015 at the Hillesden estate, Buckinghamshire, UK. Plants were genotyped at seven microsatellite markers before being introduced across the study site to form experimental arrays. Experimental arrays comprised of three E.californica plants separated by 1m and arranged in a triangular formation. A total of sixteen arrays were introduced across four 100 hectare replicate blocks, each separated by >500m. At the centre of each block, four experimental arrays were placed at 50m intervals along a 150m transect laid symmetrically across the boundary between an established wildflower patch and bare, fallow ground or grazed grassland (two arrays within the florally rich habitat and two arrays within the florally poor habitat). Upon maturation approximately 10 seeds were collected from each plant and genotyped. Fragment analysis was conducted and alleles were scored on Genemarker. Seeds were then manually scored as outcrossed or selfed and paternity was determined using Cervus. The dataset was part of a larger experiment looking at the effect of floral resources on the pollination services to isolated plants. Full details about this dataset can be found at https://doi.org/10.5285/7b721c07-bc38-4815-8669-4675867663d0

Resource type

dataset

Resource locator

https://data-package.ceh.ac.uk/sd/7b721c07-bc38-4815-8669-4675867663d0.zip

name: Supporting information

description: Supporting information available to assist in re-use of this dataset

function: information

https://data-package.ceh.ac.uk/data/7b721c07-bc38-4815-8669-4675867663d0

name: Download the data

description: Download a copy of this data

function: download

Unique resource identifier

code

https://catalogue.ceh.ac.uk/id/7b721c07-bc38-4815-8669-4675867663d0

codeSpace

doi:

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

environment

Keywords

Keyword set

keyword value

Environmental Monitoring Facilities

originating controlled vocabulary

title

reference date

date type

publication

effective date

2008-06-01

Keyword set

keyword value

originating controlled vocabulary

title

reference date

date type

creation

effective date

2012-10-29

Keyword set

keyword value

originating controlled vocabulary

title

reference date

date type

creation

effective date

2006-01-01

Keyword set

keyword value

parentage

paternity

allele

wildflower

fallow ground

grazed grassland

Californian poppy

Geographic location

West bounding longitude

-1.03

East bounding longitude

-0.965

North bounding latitude

51.979

South bounding latitude

51.936

Temporal reference

Temporal extent

Begin position

2015-06-01

End position

2015-06-30

Dataset reference date

date type

publication

effective date

2017-06-13

date type

creation

effective date

2015-06-30

Frequency of update

notPlanned

Quality and validity

Lineage

All open flowers were removed from the E. californica plants prior to their placement in pre-marked locations across the landscape. One bud on each plant was covered in a fine muslin to represent a 'pollinator excluded' flower. Plants remained in the field for 16 days to ensure full anthesis of new flowers and to allow for multiple pollination events. After this period, all fruit were tagged to ensure that only fruit development arising from the period of the field experiment were included in analyses. Plants were then collected and stored under controlled glasshouse conditions until fruit maturation. Ten progeny per plant from each of the field exposed plants were genotyped using 50mg of fresh leaf material and following standardised protocols. The incidence of self-fertilisation in plants from each habitat was calculated manually by individually comparing each successfully amplified progeny against their maternal plant. If, at each of the seven loci, the progeny was a complete match for the maternal genotype, or was homozygote for one of the maternal plants alleles, it was scored as selfed. Alternatively, if any novel alleles were observed in the progeny that were not present in the maternal plant, the progeny was classified as outcrossed. Paternity was determined using Cervus 3.0.7, where each progeny sample was listed detailing alleles at the seven microsatellite loci, specifying the known maternal sample as well as the potential paternal samples.

Conformity

Conformity report

specification

title

reference date

date type

publication

effective date

2010-12-08

degree

explanation

Data format

name of format

Comma-separated values (CSV)

version of format

Constraints related to access and use

Constraint set

Limitations on public access

Constraint set

Limitations on public access

Constraint set

Limitations on public access

© UK Centre for Ecology & Hydrology

Constraint set

Limitations on public access

If you reuse this data, you should cite: Evans, T.M., Heard, M.S., Vanbergen, A.J., Cavers, S., Ennos, R. (2017). Paternity of Eschscholzia californica plants introduced to habitats comprising different floral cover. NERC Environmental Information Data Centre https://doi.org/10.5285/7b721c07-bc38-4815-8669-4675867663d0

Responsible organisations

Responsible party

organisation name

UK Centre for Ecology & Hydrology

email address

enquiries@ceh.ac.uk

responsible party role

pointOfContact

Responsible party

organisation name

Centre for Ecology & Hydrology

email address

enquiries@ceh.ac.uk

responsible party role

author

Responsible party

organisation name

Centre for Ecology & Hydrology

email address

enquiries@ceh.ac.uk

responsible party role

author

Responsible party

organisation name

Centre for Ecology & Hydrology

email address

enquiries@ceh.ac.uk

responsible party role

author

Responsible party

organisation name

Centre for Ecology & Hydrology

email address

enquiries@ceh.ac.uk

responsible party role

author

Responsible party

organisation name

University of Edinburgh

email address

rennos@ed.ac.uk

responsible party role

author

Responsible party

organisation name

NERC Environmental Information Data Centre

email address

info@eidc.ac.uk

responsible party role

publisher

Responsible party

organisation name

Environmental Information Data Centre

email address

info@eidc.ac.uk

responsible party role

custodian

Responsible party

organisation name

UK Centre for Ecology & Hydrology

email address

enquiries@ceh.ac.uk

responsible party role

owner

Metadata on metadata

Metadata point of contact

organisation name

Environmental Information Data Centre

full postal address

Lancaster Environment Centre, Library Avenue, Bailrigg

Lancaster

LA1 4AP

UK

email address

info@eidc.ac.uk

responsible party role

pointOfContact

Metadata date

2020-03-13T14:34:53

Metadata language