Identification

Title

Soil microbial potential enzyme activities in fertilised plots in the Central Amazon, 2018

Abstract

The data consists of potential activity of enzymes released by microorganisms in soils of old growth forests in the Central Amazon. Soils were sampled in January 2018, seven months after nutrient addition in a large-scale nutrient fertilisation experiment. Enzyme activities are given as a mean of the microbial community per plot, where five points inside each plot were sampled in the 0-5 cm and 5-10 cm soil layers. The dataset depicts the potential activities of the following enzymes: ÃŸ-glucosidase, N-acetyl ÃŸ-glucosaminidase and phosphomonoesterase at two different soil depths. Samples were collected at the AFEX project area in Manaus, Brazil at the Biological Dynamics of Forest Fragments Project (BDFFP/ INPA). The study was funded by NERC, BDFFP (logistical support) and Brazilian government (student scholarship). Full details about this dataset can be found at https://doi.org/10.5285/b9a2523e-09ba-43c7-acfa-a894a231b133

Resource type

dataset

Resource locator

https://data-package.ceh.ac.uk/data/b9a2523e-09ba-43c7-acfa-a894a231b133

name: Download the data

description: Download a copy of this data

function: download

https://data-package.ceh.ac.uk/sd/b9a2523e-09ba-43c7-acfa-a894a231b133.zip

name: Supporting information

description: Supporting information available to assist in re-use of this dataset

function: information

Unique resource identifier

code

https://catalogue.ceh.ac.uk/id/b9a2523e-09ba-43c7-acfa-a894a231b133

codeSpace

doi:

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

biota

environment

Keywords

Keyword set

keyword value

Soil

originating controlled vocabulary

title

GEMET - INSPIRE themes, version 1.0

reference date

date type

publication

effective date

2008-06-01

Keyword set

keyword value

Geographic location

West bounding longitude

-68.5

East bounding longitude

-31.7

North bounding latitude

5.1

South bounding latitude

-24.6

Temporal reference

Temporal extent

Begin position

2018-01-01

End position

2018-09-30

Dataset reference date

date type

publication

effective date

2020-04-02

Frequency of update

Quality and validity

Lineage

Potential activities of 4-Methylumbelliferyl-Î²-D-glucopyranoside (BG), 4-Methylumbelliferyl-N-acetyl-Î²-D-glucosaminide (NAG) and 4-Methylumbelliferyl-phosphate (PHOS), enzymes involved in the cycling of carbon, nitrogen and phosphorus were measured using microplate fluorimetric assays. After centrifugation with buffer solution, samples were incubated for one hour using 4-Methylumbelliferyl-Î²-D-glucopyranoside (BG), 4-Methylumbelliferyl-N-acetyl-Î²-D-glucosaminide (NAG) and 4-Methylumbelliferyl-phosphate (PHOS) as substrates. Fluorescence was read on a fluorometer (Tecan InfiniteÂ® 200 PRO, GrÃ¶dig, Austria), at 365 nm excitation and 450 nm emission. Enzyme activities are presented in nmol MUF g soil dry weight-1 hour-1.

Conformity

Conformity report

specification

title

Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services

If you reuse this data, you should cite: Lugli, L.F., Quesada, C.A.N., Andersen, K.M., Mercado, L. M., Di Ponzio, R., Ribeiro, G., Assis, R. L. , Hartley, I.P. (2020). Soil microbial potential enzyme activities in fertilised plots in the Central Amazon, 2018. NERC Environmental Information Data Centre https://doi.org/10.5285/b9a2523e-09ba-43c7-acfa-a894a231b133

custodian

Metadata on metadata

Metadata point of contact

organisation name

Environmental Information Data Centre

full postal address

Lancaster Environment Centre, Library Avenue, Bailrigg

Lancaster

LA1 4AP

email address

info@eidc.ac.uk

responsible party role

pointOfContact

Metadata date

2020-04-03T07:28:38

Metadata language

eng