Identification

Title

Molecular characterization of an Endozoicomonas-like species associated with mass mortalities of king scallop Pecten maximus L. in United Kingdom

Alternative title(s)

Metagenomic sequencing of King scallop (Pecten maximus) gills infected with Endozoicomonas sp.-like organisms

Abstract

The king scallop Pecten maximus L. is the fastest growing fishery in the UK and currently the second most valuable. Mass mortality events in scallops have been reported associated with rickettsia-like organisms (RLOs) world-wide, however the causative agents were uncharacterised. In May 2013 and 2014 two mass mortality events affecting king scallops were recorded in the Lyme Bay Marine Protected Area (MPA) South West England. Histopathological examination showed gill epithelial tissue infected with RLO at varying intensity. Ultrastructural examination confirmed the intracellular location in affected epithelial cells. Partial 16S rDNA sequences of a putative RLO obtained from infected king scallop gill samples collected from both mortality events were identical and had 99.4% identity to 16S rDNA sequences obtained from Candidatus endonucleobacter bathymodioli and 95% with Endozoicomonas spp. In situ hybridisation assays using 16S rDNA probes confirmed the presence of the sequenced RLO gene in the gill tissues. Additional DNA sequences of the bacterium were obtained using high-throughput (Illumina) sequencing and bioinformatic analysis identified over 1000 genes with high similarity, ranging from 77-87% identity, to genes from Endozoicomonas spp.

Resource type

dataset

Resource locator

https://data.cefas.co.uk/view/18522

name: Cefas Data Portal

description: The Cefas Data Portal contains metadata records and data sets available to download and connect to in support of our commitment to open science. Data is available in the following formats: Binary download.

function: download

Unique resource identifier

code

CEFAS18522

codeSpace

https://data.cefas.co.uk

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

biota

Keywords

Keyword set

keyword value

originating controlled vocabulary

title

reference date

date type

publication

effective date

2008-06-01

Keyword set

keyword value

originating controlled vocabulary

title

reference date

date type

revision

effective date

2011-03-25

Keyword set

keyword value

originating controlled vocabulary

title

reference date

date type

publication

effective date

2008-06-01

Keyword set

keyword value

originating controlled vocabulary

title

reference date

date type

revision

effective date

2010-05-18

Keyword set

keyword value

originating controlled vocabulary

title

reference date

date type

publication

effective date

2012-01-11

Keyword set

keyword value

originating controlled vocabulary

title

reference date

date type

publication

effective date

2012-01-11

Geographic location

West bounding longitude

-3.50

East bounding longitude

-2.00

North bounding latitude

50.75

South bounding latitude

50.25

Temporal reference

Temporal extent

Begin position

2015-05

End position

2015-05

Dataset reference date

date type

publication

effective date

2017-05-19

date type

revision

effective date

2024-10-03

date type

creation

effective date

2017-05-05

Frequency of update

notPlanned

Quality and validity

Lineage

Specific PCR assays were developed and applied to screen for the presence of 16S rDNA RLO sequences in king scallop gill tissues collected in the Lyme Bay MPA during 2015. There was 100% prevalence of the RLO in these gill tissues and the 16S rDNA sequences identified were identical to the sequence found during the previous mortality event.

Conformity

Conformity report

specification

title

reference date

date type

publication

effective date

2013-12-10

degree

false

explanation

See the referenced specification

Conformity report

specification

title

reference date

date type

publication

effective date

2010-12-08

degree

true

explanation

See the referenced specification

Data format

name of format

Unknown

version of format

Constraints related to access and use

Constraint set

Limitations on public access

Constraint set

Limitations on public access

Responsible organisations

Responsible party

organisation name

Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS)

full postal address

Cefas Lowestoft Laboratory

Pakefield Road

Lowestoft

NR33 0HT

UK

email address

data.manager@cefas.co.uk

responsible party role

originator

Responsible party

organisation name

Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS)

full postal address

Cefas Lowestoft Laboratory

Pakefield Road

Lowestoft

NR33 0HT

UK

email address

data.manager@cefas.co.uk

responsible party role

custodian

Responsible party

organisation name

Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS)

full postal address

Cefas Lowestoft Laboratory

Pakefield Road

Lowestoft

NR33 0HT

UK

email address

data.manager@cefas.co.uk

responsible party role

distributor

Metadata on metadata

Metadata point of contact

organisation name

Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS)

full postal address

Cefas Lowestoft Laboratory

Pakefield Road

Lowestoft

NR33 0HT

UK

email address

data.manager@cefas.co.uk

responsible party role

pointOfContact

Metadata date

2024-10-03T08:57:34

Metadata language