Operational taxonomic units for soil bacteria in the Wolf and Tamar catchments
This dataset contains operational taxonomic units for soil bacteria collected from various land use categories in the Wolf and Tamar catchments in South west England. A range of soils were targeted from the Tamar region comprising a range of land uses. Approximate location of sampling sites was determined from maps to provide good spatial coverage of the catchment. Exact sites were determined in the field, considering accessibility and other logistic, and soils taken. Full details about this dataset can be found at https://doi.org/10.5285/296ded8e-2c80-4a01-98cc-e71e3fa3fa1b
dataset
https://data-package.ceh.ac.uk/sd/296ded8e-2c80-4a01-98cc-e71e3fa3fa1b.zip
name: Supporting Information
description: Supporting information available to assist in re-use of this dataset
function: information
https://data-package.ceh.ac.uk/data/296ded8e-2c80-4a01-98cc-e71e3fa3fa1b
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1395928628829
CEH:EIDC:
eng
biota
environment
inlandWaters
location
microbiology
genetic information
Tellus
South West project
-6.452
1.768
55.812
49.864
2013-10-01
2014-03-20
publication
2014-05-06
unknown
Epilithon (water samples): Approximate location of sampling sites was determined from maps to provide good spatial coverage of the Wold River through to the Tamar River. Exact sites were determined in the field, considering accessibility and other logistics. The exact location of each sample site was determined using a Garmin GPS12. Three stones were taken from each of the 20 locations and epilithon removed from a defined area. Samples were kept in the cold and removed to the laboratory for analyses. DNA was extracted from all soil and epilithon samples using the MOBIO Powersoil 96 well DNA extraction kit. DNA was quality checked for purity and yield prior to submission for 454 pyrosequencing to assess both bacterial and eukaryotic biodiversity within each sample. Following bioinformatic sequence processing, sequencing were clustered into operational taxonomic units (OTU) and the data tables display the percentage of each OTU within each sample.
publication
2010-12-08
Comma-separated values (CSV)
© UK Centre for Ecology & Hydrology
If you reuse this data, you should cite: Griffiths, R., Pywell, R.F., Peyton, J., Nuttall, P., Sarlej, R., Thomson, B. (2014). Operational taxonomic units for soil bacteria in the Wolf and Tamar catchments. NERC Environmental Information Data Centre https://doi.org/10.5285/296ded8e-2c80-4a01-98cc-e71e3fa3fa1b
UK Centre for Ecology & Hydrology
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Centre for Ecology & Hydrology
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Centre for Ecology & Hydrology
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Centre for Ecology & Hydrology
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Centre for Ecology & Hydrology
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Centre for Ecology & Hydrology
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Centre for Ecology & Hydrology
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Centre for Ecology & Hydrology
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NERC Environmental Information Data Centre
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Environmental Information Data Centre
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UK Centre for Ecology & Hydrology
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Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg
Lancaster
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UK
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2020-03-13T14:27:19