Identification

Title

Heterogeneity and environmental stress resistance of wild yeast isolates

Abstract

[This dataset is embargoed until August 31, 2020]. This dataset contains analytical results for wild yeast isolates from soil samples at and near a disused metal smelting works in the north-east of the UK. The main contaminant from the smelting works was lead (Pb), but Cd was also elevated. The soil near the smelting works showed above-background levels of Pb. Yeasts of similar colony morphology on laboratory agar were isolated and proved to be mostly isolates of Saitozyma podzolica: 56 independent clonal isolates of this yeast were confirmed by ITS sequencing and RAPD-PCR. All of the S. podzolica isolates were tested for Pb and Cd resistance (IC50) and heteroresistance. Heteroresistance indicated variation in metal resistance between individual cells of clonal populations. Some isolates were also tested with Zn. The genomes of the isolates were sequenced and sequence data submitted to the NCBI/EBI. Full details about this dataset can be found at https://doi.org/10.5285/299f89cd-7bbe-4428-9552-c51981c5f883

Resource type

dataset

Resource locator

Unique resource identifier

code

https://catalogue.ceh.ac.uk/id/299f89cd-7bbe-4428-9552-c51981c5f883

codeSpace

doi:

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

biota

Keywords

Keyword set

keyword value

Soil

originating controlled vocabulary

title

GEMET - INSPIRE themes, version 1.0

reference date

date type

publication

effective date

2008-06-01

Keyword set

keyword value

Wild Yeast

Environmental stress resistance

Heterogeneity

Geographic location

West bounding longitude

-1.589

East bounding longitude

-1.574

North bounding latitude

54.983

South bounding latitude

54.972

Temporal reference

Temporal extent

Begin position

2014-07-01

End position

2014-07-31

Dataset reference date

date type

publication

effective date

2019-10-10

Frequency of update

Quality and validity

Lineage

Soil-core samples were collected during July 2014 near the disused St. Anthonyâ€™s metal smelting works, at either contaminated (16.03 g kg-1 Pb, 10.87 mg Kg-1 Cd) or nearby less-contaminated (4.38 g kg-1 Pb, 1.49 mg Kg-1 Cd) areas. Coordinates and a description of the site are available at http://www.twsitelines.info/SMR/4192. Soil-core samples were diluted in the laboratory and spread to agar media for yeast isolation. Single colonies were subcultured and yeasts identified with ITS sequencing and RAPD PCR. 56 clonal isolates of Saitozyma podzolica were assayed for lead (Pb) and cadmium (Cd) resistance (IC50) and heteroresistance. Some isolates were also tested with zinc (Zn) Genomic DNA preparations submitted to NBAF-Liverpool for whole-genome sequencing. The sequence data were deposited with EBI/NCBI.

Conformity

Conformity report

specification

title

Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services

If you reuse this data, you should cite: Hewitt, S., Warry, A., Dyer, P.S., Avery, S.V. (2019). Heterogeneity and environmental stress resistance of wild yeast isolates. NERC Environmental Information Data Centre https://doi.org/10.5285/299f89cd-7bbe-4428-9552-c51981c5f883

pointOfContact

Metadata on metadata

Metadata point of contact

organisation name

Environmental Information Data Centre

full postal address

Lancaster Environment Centre, Library Avenue, Bailrigg

Lancaster

LA1 4AP

email address

info@eidc.ac.uk

responsible party role

pointOfContact

Metadata date

2020-03-13T14:23:47

Metadata language

eng