Identification

Title

Molecular analysis of Glossina morsitans morsitans tsetse from Mambwe District, Zambia (2013)

Abstract

This data set describes the prevalence of trypanosomes and Sodalis glossinidius, and host blood meal analysis from tsetse flies (Glossina morsitans morsitans) captured during two intensive surveys in Mambwe District, Eastern Province, Zambia in 2013. The Luangwa Valley in Zambia is an old focus of human African trypanosomiasis (HAT, Trypanosoma brucei rhodesiense) and sporadic outbreaks have continued to occur in the human population. In recent years there has been an influx of people migrating from the densely populated plateau region resulting in a significant change in land-use in the study area, potentially influencing tsetse dynamics and the epidemiology of HAT. This data set was collected to monitor infection rates of trypanosomes and Sodalis glossinidius in Glossina morsitans morsitans tsetse flies in the area so as to assess the risk posed to both human and livestock populations. In addition, feeding patterns of tsetse were investigated through analysis of blood meals. This work was part of a wider research project, the Dynamic Drivers of Disease in Africa Consortium (DDDAC) and contributed to the Zambia trypanosomiasis case study. The research was funded by NERC project no NE/J000701/1 with support from the Ecosystem Services for Poverty Alleviation Programme (ESPA). Full details about this dataset can be found at https://doi.org/10.5285/a55eea77-8401-49ba-921e-53e085dc8345

Resource type

dataset

Resource locator

https://data-package.ceh.ac.uk/data/a55eea77-8401-49ba-921e-53e085dc8345

name: Download the data

description: Download a copy of this data

function: download

https://data-package.ceh.ac.uk/sd/a55eea77-8401-49ba-921e-53e085dc8345.zip

name: Supporting information

description: Supporting information available to assist in re-use of this dataset

function: information

Unique resource identifier

code

https://catalogue.ceh.ac.uk/id/a55eea77-8401-49ba-921e-53e085dc8345

codeSpace

doi:

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

biota

health

Keywords

Keyword set

keyword value

originating controlled vocabulary

title

Wikidata

reference date

date type

creation

effective date

2012-10-29

Keyword set

keyword value

originating controlled vocabulary

title

GeoNames

reference date

date type

creation

effective date

2006-01-01

Keyword set

keyword value

Blood meal

Tsetse

trypanosome

Dynamic Drivers of Disease in Africa Consortium (DDDAC)

Luangwa Valley

Geographic location

West bounding longitude

31.826

East bounding longitude

32.094

North bounding latitude

-13.248

South bounding latitude

-13.828

Temporal reference

Temporal extent

Begin position

2013-05-01

End position

2013-11-30

Dataset reference date

date type

publication

effective date

2017-03-24

date type

creation

effective date

2016-11-21

Frequency of update

Quality and validity

Lineage

Tsetse surveys were conducted in May / June 2013 and November 2013. Tsetse flies were sampled using black screen fly rounds. Data were recorded on standard field sampling forms and then manually transferred to Microsoft Excel. Data were then checked for accuracy and incorrect spatial location coordinates removed. Tsetse flies were homogenized and DNA extracted. The resulting DNA was stored at minus 20 degrees Celsius until needed for Polymerase chain reaction (PCR). PCR analysis was performed using different primers to detect the different species of trypanosomes in the DNA. All PCR included a negative and a positive control. To determine the sources of blood meal (feeding patterns), tsetse DNA samples were subjected to PCR targeting the cytochrome oxidase b gene. The resulting PCR product was sequenced, and the sequences compared with those available in public databases by using nucleotide BLAST at NCBI website (http://blast.ncbi.nlm.nih.gov/Blast.cgi) to determine the animal species on which tsetse fed on. Data were collated in a Microsoft Excel spreadsheet and exported as a comma separated file for ingestion into the Environmental Information Data Centre (EIDC).

Conformity

Conformity report

specification

title

Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services

reference date

date type

publication

effective date

2010-12-08

degree

explanation

Data format

name of format

Comma-separated values (CSV)

version of format

Constraints related to access and use

Constraint set

Limitations on public access

Constraint set

Limitations on public access

Constraint set

Limitations on public access

Constraint set

Limitations on public access

Constraint set

Limitations on public access

If you reuse this data, you should cite: Simuunza, M., Anderson, N.E, Machila, N., Ndebe, J. , Macleod, E.T., Welburn, S.C. (2017). Molecular analysis of Glossina morsitans morsitans tsetse from Mambwe District, Zambia (2013). NERC Environmental Information Data Centre https://doi.org/10.5285/a55eea77-8401-49ba-921e-53e085dc8345

publisher

Metadata on metadata

Metadata point of contact

organisation name

Environmental Information Data Centre

full postal address

Lancaster Environment Centre, Library Avenue, Bailrigg

Lancaster

LA1 4AP

email address

info@eidc.ac.uk

responsible party role

pointOfContact

Metadata date

2022-05-20T10:39:28

Metadata language

eng