Identification

Title

Intra-crystalline protein degradation dating of proboscidean enamel from the UK (NERC Grant NE/K500987/1)

Abstract

The concentration of both L and D amino acid isomers was determined by HPLC (High-performance liquid chromatography) for multiple amino acids. 30 samples of proboscidean enamel were taken from the collections at BGS. Samples of enamel (~30 mg) was removed from the teeth of proboscideans using a small electric drill. All of the samples were from UK sites dating to the Quaternary. These samples were used to build a regional geochronology based on the extent of racemisation of amino acids from the intra-crystalline fraction (Dickinson, 2018). Thesis http://etheses.whiterose.ac.uk/22261. A published paper 'A new method for enamel amino acid racemization dating: A closed system approach' is also available; https://doi.org/10.1016/j.quageo.2018.11.005

Resource type

dataset

Resource locator

http://etheses.whiterose.ac.uk/22261

function: information

https://doi.org/10.1016/j.quageo.2018.11.005

function: information

https://webapps.bgs.ac.uk/services/ngdc/accessions/index.html#item139845

name: Data

function: download

Unique resource identifier

code

http://data.bgs.ac.uk/id/dataHolding/13607694

codeSpace

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

geoscientificInformation

Keywords

Keyword set

keyword value

originating controlled vocabulary

title

reference date

date type

publication

effective date

2008-06-01

Keyword set

keyword value

Geochronology

Racemization dating

Age determination methods

originating controlled vocabulary

title

reference date

date type

revision

effective date

2011

Keyword set

keyword value

NERC_DDC

Geographic location

West bounding longitude

0.0700

East bounding longitude

1.7000

North bounding latitude

52.9200

South bounding latitude

51.4400

Extent

Extent group

authority code

title

reference date

date type

revision

effective date

2009

code identifying the extent

ENG

Extent group

authority code

title

reference date

date type

creation

effective date

1979

code identifying the extent

ENGLAND [id=150000]

Temporal reference

Temporal extent

Begin position

2017-05-08

End position

2018-07-01

Dataset reference date

date type

creation

effective date

2020-11-27

Frequency of update

notApplicable

Quality and validity

Lineage

Samples of enamel (~30 mg) were powdered and exposed to NaOCl (12%) for 72 h. The NaOCl was removed and the powdered enamel was washed 5X with water and 1X with MeOH. Powdered enamel samples were split into 2 fractions. One sample was treated for their free amino acid (FAA) content and the other their total hydrolysable amino acid (THAA) content. THAA samples were dissolved in HCl (7M) and heated at 110 ̊C for 24 h. THAA samples were re-dissolved in HCl (1 M, 20 μLmg−1) and FAA samples were demineralised in HCl (1 M, 25 μLmg−1). KOH (1 M, 28 μLmg−1) was added to the acidified solutions. The sample was centrifuged for 10 min causing a clear supernatant to form above a gel; the biphasic separation. The supernatant was extracted and dried by centrifugal evaporation and analysed by HPLC. All methods are outlined in Dickinson et al., 2019.

Conformity

Conformity report

specification

title

reference date

date type

publication

effective date

2011

degree

false

explanation

See the referenced specification

Conformity report

specification

title

reference date

date type

publication

effective date

2010-12-08

degree

false

explanation

See http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2010:323:0011:0102:EN:PDF

Data format

name of format

MS Excel

version of format

Constraints related to access and use

Constraint set

Limitations on public access

Constraint set

Limitations on public access

The copyright of materials derived from the British Geological Survey's work is vested in the Natural Environment Research Council [NERC]. No part of this work may be reproduced or transmitted in any form or by any means, or stored in a retrieval system of any nature, without the prior permission of the copyright holder, via the BGS Intellectual Property Rights Manager. Use by customers of information provided by the BGS, is at the customer's own risk. In view of the disparate sources of information at BGS's disposal, including such material donated to BGS, that BGS accepts in good faith as being accurate, the Natural Environment Research Council (NERC) gives no warranty, expressed or implied, as to the quality or accuracy of the information supplied, or to the information's suitability for any use. NERC/BGS accepts no liability whatever in respect of loss, damage, injury or other occurence however caused.

Responsible organisations

Responsible party

contact position

Chemistry Department

organisation name

University of York

full postal address

York

YO10 5DD

email address

not available

responsible party role

originator

Responsible party

organisation name

British Geological Survey

full postal address

Environmental Science Centre, Nicker Hill, Keyworth

NOTTINGHAM

NG12 5GG

United Kingdom

telephone number

0115 936 3143

facsimile number

0115 936 3276

email address

enquiries@bgs.ac.uk

responsible party role

distributor

Responsible party

organisation name

British Geological Survey

full postal address

Environmental Science Centre, Nicker Hill, Keyworth

NOTTINGHAM

NG12 5GG

United Kingdom

telephone number

0115 936 3143

facsimile number

0115 936 3276

email address

enquiries@bgs.ac.uk

responsible party role

pointOfContact

Metadata on metadata

Metadata point of contact

organisation name

British Geological Survey

full postal address

Environmental Science Centre,Keyworth

NOTTINGHAM

NG12 5GG

United Kingdom

telephone number

+44 115 936 3100

email address

enquiries@bgs.ac.uk

responsible party role

pointOfContact

Metadata date

2022-01-19

Metadata language