Time series of microbial carbon release from soil as carbon dioxide under different nitrogen and phosphorus treatments with a high molecular weight substrate added as a carbon source, Conwy catchment, North Wales, UK (2016)
Time series data of carbon release in disintegrations per minute are presented for different nitrogen and phosphorus treatments with a high molecular weight substrate added as a carbon source to soil samples from six depths (0-15, 15-30, 50-100, 100-150, 150-200 and 250-300 centimetres). Soil cores were collected from a field experiment in the Conwy catchment in July 2016 and returned the laboratories of the School of Environment, Natural Resources and Geography, Bangor University. A high molecular weight substrate was added as a carbon source to the samples and the rate of 14C-substrate mineralization measured. All the work was carried out by trained members of staff from Bangor University and the Centre for Ecology & Hydrology. The measurements were taken to improve understanding of the relationship between microbial activity and soil properties and depth of sampling, under differing nutrient availability. The data were collected for the NERC project 'The Multi-Scale Response of Water quality, Biodiversity and Carbon Sequestration to Coupled Macronutrient Cycling from Source to Sea' (NE/J011991/1). The project is also referred to as Turf2Surf. Full details about this dataset can be found at https://doi.org/10.5285/4a8fedf4-a572-4d95-ac41-087974169af1
dataset
https://data-package.ceh.ac.uk/sd/4a8fedf4-a572-4d95-ac41-087974169af1.zip
name: Supporting information
description: Supporting information available to assist in re-use of this dataset
function: information
name: Download the data
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https://catalogue.ceh.ac.uk/id/4a8fedf4-a572-4d95-ac41-087974169af1
doi:
eng
environment
Environmental Monitoring Facilities
publication
2008-06-01
T2S
Turf2Surf
Macronutrient Cycling Programme
MCP
North Wales
Conwy catchment
liquid scintillation counter
Soil Core
-3.784
-3.781
53.204
53.201
2016-07-15
2016-09-30
publication
2018-03-20
creation
2016-11-30
notPlanned
Soil cores were collected from the field site and returned to laboratories of the School of Environment, Natural Resources and Geography, Bangor University. In the laboratory soil cores were divided into depth intervals of 0-15, 15-30, 50-100, 100-150, 150-200 and 250-300 centimetres and passed through a 5 millimetre sieve in order to remove stones and any plant material and to ensure sample homogeneity. To measure the rate of 14C-substrate mineralization, 5 grams of soil (dry weight equivalent to account for soil water content variability down the soil profile) was placed into sterile 50ml polypropylene tubes. To determine the rate of 14CO2 evolution 160µl of the recalcitrant 14C-DOC labelled nutrient solution was added to the soil surface. Immediately after nutrient addition, a vial containing 1 millilitre (ml) Sodium Hydroxide (NaOH) trap (1 Molar (M)) was added into the polypropylene tubes to capture 14CO2 evolved. The tubes were hermetically sealed and incubated at 10 degrees Celsius to represent the mean annual temperature of the catchment. The NaOH traps were changed at 1, 6, 24, 48, 72, 168, 336, 504, 672, 840, 1176, 1512 and 1680 hours. On removal, the NaOH traps were mixed with Optiphase HiSafe 3® liquid scintillation fluid (PerkinElmer Inc.) and the amount of 14CO2 captured was determined using a Wallac 1404 liquid scintillation counter (Wallac EG & G). Data were entered into an Excel spreadsheet and exported as a .csv file for ingestion into the EIDC.
publication
2010-12-08
Comma-separated values (CSV)
© UK Centre for Ecology & Hydrology
© Bangor University
If you reuse this data, you should cite: Glanville, H.C., De Sosa, L.L. , Marshall, M.R., Cooper, D.M., Jones, D.L. (2018). Time series of microbial carbon release from soil as carbon dioxide under different nitrogen and phosphorus treatments with a high molecular weight substrate added as a carbon source, Conwy catchment, North Wales, UK (2016). NERC Environmental Information Data Centre https://doi.org/10.5285/4a8fedf4-a572-4d95-ac41-087974169af1
Bangor University
author
Bangor University
author
Centre for Ecology & Hydrology
author
Centre for Ecology & Hydrology
author
Bangor University
author
NERC EDS Environmental Information Data Centre
custodian
NERC Environmental Information Data Centre
publisher
Bangor University
pointOfContact
Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg
Lancaster
LA1 4AP
UK
pointOfContact
2021-06-25T18:43:43