Identification

Title

The amino acid composition of 20 aragonite samples precipitated in vitro in the presence and absence of amino acid (aspartic acid and glycine, separately and in combination), the dipeptide glycyl-L-aspartic acid and tetra-aspartic acid.

Abstract

Amino acid compositions of aragonite samples precipitated from seawater, using a pH stat titrator using the constant composition technique between September 2021 and December 2022. Samples were precipitated from 330 mL of seawater with no biomolecules (control) or with a seawater concentration of 2 mM of aspartic acid (Asp), 2 mM glycine (Gly), 2 mM of both amino acids (Asp+Gly) or 2 mM dipeptide glycyl-L-aspartic acid (Asp-Gly) or from 33 mL of seawater with variable concentrations of aspartic acid (Asp) or tetra-aspartic acid (Asp4). Protein was extracted from the samples and run as free amino acids (to detect amino acids in free form) and as hydrolysed samples (to detect peptides). Data were collected to determine how changes in the calcification fluids of calcareous organisms affect aragonite precipitation. Data were collected by Giacomo Gardella, Sam Presslee and Nicola Allison and interpreted by Giacomo Gardella, Cristina Castillo Alvarez, Nicola Allison, Adrian Finch, Kirsty Penkman, Roland Krӧger, Sam Presslee and Matthieu Clog

Resource type

nonGeographicDataset

Resource locator

https://webapps.bgs.ac.uk/services/ngdc/accessions/index.html#item186472

name: Data

function: download

Unique resource identifier

code

http://data.bgs.ac.uk/id/dataHolding/13608300

codeSpace

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

geoscientificInformation

Keywords

Keyword set

keyword value

originating controlled vocabulary

title

reference date

date type

publication

effective date

2008-06-01

Keyword set

keyword value

NGDC Deposited Data

Calcification

Aragonite

Sea water

originating controlled vocabulary

title

reference date

date type

revision

effective date

2022

Keyword set

keyword value

Keyword set

keyword value

NERC_DDC

Geographic location

West bounding longitude

East bounding longitude

North bounding latitude

South bounding latitude

Temporal reference

Temporal extent

Begin position

2021-09-01

End position

2022-12-23

Dataset reference date

date type

creation

effective date

2024-10-30

Frequency of update

notApplicable

Quality and validity

Lineage

Artificial seawater was manipulated to alter pH and dissolved inorganic carbon concentration and the biomolecule, if used, was added. An aragonite seed was added as a surface for aragonite growth. A pH stat titrator monitored pH and dosed titrants (Na2CO3 and CaCl2) to replace the Ca2+ and CO32- consumed during aragonite formation. At the end, the solid was collected by filtration, rinsed with water and ethanol, dried at 40°C and stored. For amino acid analysis, samples were bleached, rinsed, demineralised and characterised by reverse phase HPLC in both free and hydrolysed form.

Conformity

Conformity report

specification

title

reference date

date type

publication

effective date

2011

degree

false

explanation

See the referenced specification

Conformity report

specification

title

reference date

date type

publication

effective date

2010-12-08

degree

false

explanation

See http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2010:323:0011:0102:EN:PDF

Data format

name of format

CSV

version of format

Constraints related to access and use

Constraint set

Limitations on public access

Constraint set

Limitations on public access

The copyright of materials derived from the British Geological Survey's work is vested in the Natural Environment Research Council [NERC]. No part of this work may be reproduced or transmitted in any form or by any means, or stored in a retrieval system of any nature, without the prior permission of the copyright holder, via the BGS Intellectual Property Rights Manager. Use by customers of information provided by the BGS, is at the customer's own risk. In view of the disparate sources of information at BGS's disposal, including such material donated to BGS, that BGS accepts in good faith as being accurate, the Natural Environment Research Council (NERC) gives no warranty, expressed or implied, as to the quality or accuracy of the information supplied, or to the information's suitability for any use. NERC/BGS accepts no liability whatever in respect of loss, damage, injury or other occurence however caused.

Responsible organisations

Responsible party

organisation name

University of St Andrews

email address

not available

responsible party role

originator

Responsible party

organisation name

British Geological Survey

email address

not available

responsible party role

distributor

Responsible party

organisation name

British Geological Survey

email address

not available

responsible party role

pointOfContact

Metadata on metadata

Metadata point of contact

organisation name

British Geological Survey

full postal address

Environmental Science Centre,Keyworth

NOTTINGHAM

NG12 5GG

United Kingdom

telephone number

+44 115 936 3100

email address

enquiries@bgs.ac.uk

responsible party role

pointOfContact

Metadata date

2025-03-10

Metadata language