Wild rodent trapping, tracking and sampling for genetics and gut microbiome, Silwood Park, Berkshire, November 2014 to November 2015
This data set is a combination of trapping data, tracking data and data on the genetic relatedness and gut microbiome composition of wild rodents caught in 2.3 ha study site in Nash’s Copse, Silwood Park, from November 2014 to November 2015. As part of a longer-term capture-mark-recapture study, three species of rodents were trapped with Sherman live-traps fortnightly for 12 months: wood mouse (Apodemus sylvaticus), yellow-necked mouse (Apodemus flavicollis) and bank vole (Myodes glaerolus). Upon capture, they were measured, weighted, sexed, aged and a tissue sample and a faecal sample were collected from all mouse individuals for genetic and microbiome analyses. All rodents were released to their location of capture. First time each individual was captured, they were injected with a permanent subcutaneous identification Radio-Frequency Identification(RFID)-tag (Passive Integrated Transponde-tag). The tagged rodents were subsequently tracked with a set of custom-made tracking devices (loggers). Loggers produced dense time-stamped occurrence data suitable for inferring spatio-temporal activity patterns of rodents, such as temporal niches, home ranges and social networks. Tissue samples were used to genotype the wood mouse population and bacterial DNA extracted from faecal samples were used to profile their gut microbiome composition. This work was funded by a NERC independent Research Fellowship to Sarah Knowles (NE/L011867/1) Full details about this dataset can be found at https://doi.org/10.5285/c67fde7f-a1c8-4cb4-a76e-0c6d21c82222
dataset
https://data-package.ceh.ac.uk/data/c67fde7f-a1c8-4cb4-a76e-0c6d21c82222
name: Download the data
description: Download a copy of this data
function: download
https://data-package.ceh.ac.uk/sd/c67fde7f-a1c8-4cb4-a76e-0c6d21c82222.zip
name: Supporting information
description: Supporting information available to assist in re-use of this dataset
function: information
https://catalogue.ceh.ac.uk/id/c67fde7f-a1c8-4cb4-a76e-0c6d21c82222
doi:
eng
biota
Environmental Monitoring Facilities
publication
2008-06-01
Microbiome
microbiota
gut microbiome
trapping
Sherman
wild mouse
wild
mouse
wood mouse
bank vole
yellow-necked mouse
Apodemus
Myodes
RFID-tracking
social network analysis
home range analysis
microsatellites
kinship
pedigree
-0.66
-0.633
51.422
51.398
2014-11-01
2015-11-30
publication
2022-11-25
Wild rodents were trapped fortnightly, tagged and continuously monitored with tracking technology for one year. This is a natural rodent population and no experimental manipulations were made. Upon capture, faecal samples were collected from the rodents and a piece of ear tissue was collected upon first capture of each individual. Tissue samples were stored in ethanol in -20 C and faecal samples were stored without buffer in -80 C for 3 years. In 2017 DNA was extracted from the faecal samples of wood mice and their gut microbiome was characterised from this by amplification and sequencing of the V3 region of bacterial 16S rRNA marker gene. In 2018 the wood mouse population was genotyped and their pedigree reconstructed from ear tissue samples using 11 microsatellite markers. Details of the data and the cleaning process can be found from the supporting documentation.
publication
2010-12-08
Comma-separated values (CSV)
If you reuse this data, you should cite: Raulo, A., Allen, B.E., Troitsky, T., Husby, A., Firth, J., Coulson, T., Knowles, S.C.L. (2022). Wild rodent trapping, tracking and sampling for genetics and gut microbiome, Silwood Park, Berkshire, November 2014 to November 2015. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/c67fde7f-a1c8-4cb4-a76e-0c6d21c82222
University of Oxford
author
University of Liverpool
author
University of Helsinki
author
Uppsala University
author
University of Oxford
author
University of Oxford
author
University of Oxford
author
University of Oxford
pointOfContact
NERC EDS Environmental Information Data Centre
custodian
NERC EDS Environmental Information Data Centre
publisher
Imperial College London
owner
Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg
Lancaster
LA1 4AP
UK
pointOfContact
2023-02-09T11:08:57