Identification

Title

Time series of microbial carbon release from soil as carbon dioxide under different nitrogen and phosphorus treatments with a low glucose concentration added as a carbon source in the Conwy catchment, North Wales, UK (2016)

Abstract

Time series data of carbon release in disintegrations per minute are presented for different nitrogen and phosphorus treatments with a low glucose concentration substrate added as a carbon source to soil samples from six depths (0-15, 15-30, 50-100, 100-150, 150-200 and 250-300 centimetres). Soil cores were collected from a field experiment in the Conwy catchment in July 2016 and returned the laboratories of the School of Environment, Natural Resources and Geography, Bangor University. A high molecular weight substrate was added as a carbon source to the samples and the rate of 14C-substrate mineralization measured. All the work was carried out by trained members of staff from Bangor University and the Centre for Ecology & Hydrology. The measurements were taken to improve understanding of the relationship between microbial activity and soil properties and depth of sampling, under differing nutrient availability. The data were collected for the NERC project 'The Multi-Scale Response of Water quality, Biodiversity and Carbon Sequestration to Coupled Macronutrient Cycling from Source to Sea' (NE/J011991/1). The project is also referred to as Turf2Surf. Full details about this dataset can be found at https://doi.org/10.5285/c29d2ce1-0295-439b-adf1-98ed59f73fae

Resource type

dataset

Resource locator

https://data-package.ceh.ac.uk/data/c29d2ce1-0295-439b-adf1-98ed59f73fae

name: Download the data

description: Download a copy of this data

function: download

https://data-package.ceh.ac.uk/sd/c29d2ce1-0295-439b-adf1-98ed59f73fae.zip

name: Supporting information

description: Supporting information available to assist in re-use of this dataset

function: information

Unique resource identifier

code

https://catalogue.ceh.ac.uk/id/c29d2ce1-0295-439b-adf1-98ed59f73fae

codeSpace

doi:

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

environment

Keywords

Keyword set

keyword value

Environmental Monitoring Facilities

originating controlled vocabulary

title

GEMET - INSPIRE themes, version 1.0

reference date

date type

publication

effective date

2008-06-01

Keyword set

keyword value

T2S

Turf2Surf

Macronutrient Cycling Programme

MCP

North Wales

Conwy catchment

liquid scintillation counter

Soil core

Geographic location

West bounding longitude

-3.784

East bounding longitude

-3.781

North bounding latitude

53.204

South bounding latitude

53.201

Temporal reference

Temporal extent

Begin position

2016-07-15

End position

2016-09-30

Dataset reference date

date type

publication

effective date

2018-03-19

date type

creation

effective date

2016-11-30

Frequency of update

notPlanned

Quality and validity

Lineage

Soil cores were collected from the field site and returned to laboratories of the School of Environment, Natural Resources and Geography, Bangor University. In the laboratory soil cores were divided into depth intervals of 0-15, 15-30, 50-100, 100-150, 150-200 and 250-300 centimetres and passed through a 5 millimetre sieve in order to remove stones and any plant material and to ensure sample homogeneity. To measure the rate of 14C-substrate mineralization, 5 grams of soil (dry weight equivalent to account for soil water content variability down the soil profile) was placed into sterile 50 ml polypropylene tubes. To determine the rate of 14CO2 evolution, 50 microlitres (Âµl) of 14C-glucose labelled nutrient solution was added to the soil surface. Immediately after nutrient addition, a vial containing 1 millilitre (ml) Sodium Hydroxide (NaOH) trap (1 Molar (M)) was added into the polypropylene tubes to capture 14CO2 evolved. The tubes were hermetically sealed and incubated at 10 degrees Celsius to represent the mean annual temperature of the catchment. The NaOH traps were changed after 0.5, 1, 2, 4, 6, 24, 48, 72, 96, 120, 144, 168, 192, 336, 504, 672, 840 and 1008 hours and then weekly up to 6 weeks after initial 14C-labelling for the glucose-C additions. On removal, the NaOH traps were mixed with Optiphase HiSafe 3Â® liquid scintillation fluid (PerkinElmer Inc.) and the amount of 14CO2 captured was determined using a Wallac 1404 liquid scintillation counter (Wallac EG & G). Data were entered into an Excel spreadsheet and exported as a .csv file for ingestion into the EIDC

Conformity

Conformity report

specification

title

Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services

Â© UK Centre for Ecology & Hydrology

Constraint set

Limitations on public access

If you reuse this data, you should cite: Glanville, H.C., De Sosa, L.L. , Marshall, M.R., Cooper, D.M., Jones, D.L. (2018). Time series of microbial carbon release from soil as carbon dioxide under different nitrogen and phosphorus treatments with a low glucose concentration added as a carbon source in the Conwy catchment, North Wales, UK (2016). NERC Environmental Information Data Centre https://doi.org/10.5285/c29d2ce1-0295-439b-adf1-98ed59f73fae

publisher

Metadata on metadata

Metadata point of contact

organisation name

Environmental Information Data Centre

full postal address

Lancaster Environment Centre, Library Avenue, Bailrigg

Lancaster

LA1 4AP

email address

info@eidc.ac.uk

responsible party role

pointOfContact

Metadata date

2020-03-13T14:25:02

Metadata language

eng