Identification

Title

Antibiotic susceptibility tests and resistance genes in Escherichia coli from humans, poultry and environmental samples in urban and rural Bangladesh (2017)

Abstract

Antibiotic susceptibility tests are presented as the zone of inhibition using the disc-diffusion method, and categorized as resistant, intermediate or susceptible. DNA samples from antibiotic-resistant bacteria were analysed for the presence or absence of resistance genes using polymerase chain reaction (PCR). Laboratory analyses were conducted by trained staff at the International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b). The aim of the study was to identify the antibiotic-susceptibility profiles and resistance genes of bacteria (Escherichia coli) obtained from humans, poultry and the environment. Bacterial isolates previously identified with resistance to third-generation cephalosporins or carbapenems were included in the analysis. Bacterial samples originated from rural households and poultry farms (broiler chickens) in Mirzapur, Tangail district; and urban food markets in Dhaka city, Bangladesh. Environmental samples included surface water, water supply, wastewater, soil, animal faeces (poultry and cattle) and solid waste. The survey was part of a wider research project, Spatial and Temporal Dynamics of Antimicrobial Resistance Transmission from the Outdoor Environment to Humans in Urban and Rural Bangladesh. The research was funded by NERC/BBSRC/MRC on behalf of the Antimicrobial Resistance Cross-Council Initiative award NE/N019555/1.

Resource type

dataset

Resource locator

Unique resource identifier

code

https://catalogue.ceh.ac.uk/id/dda6dd55-f955-4dd5-bc03-b07cc8548a3d

codeSpace

doi:

Dataset language

eng

Spatial reference system

authority code

urn:ogc:def:crs:EPSG

code identifying the spatial reference system

4326

Classification of spatial data and services

Topic category

health

Keywords

Keyword set

keyword value

Escherichia coli

Keyword set

keyword value

antibiotic susceptibility tests

antimicrobial resistance

infectious disease

microbiology

bacterial counts

carbapenem

third-generation cephalosporin

DNA

Geographic location

West bounding longitude

87.284

East bounding longitude

98.402

North bounding latitude

29.926

South bounding latitude

20.314

Temporal reference

Temporal extent

Begin position

2017-02-01

End position

2018-10-31

Dataset reference date

date type

publication

effective date

2019-02-11

date type

creation

effective date

2018-12-15

Frequency of update

notPlanned

Quality and validity

Lineage

Water (drinking, stream, waste and pond), soil and faecal samples were collected from farms and urban markets. Laboratory analysis of bacterial samples took place at International Centre for Diarrhoeal Disease Research , Dhaka, Bangladesh. Laboratory results were recorded on paper-based forms or generated from automated readings, entered into Excel then converted to .csv files for ingestion into the Environmental Information Data Centre. Reference organisms and Clinical and Laboratory Standard Institute (CLSI) guidelines were followed.

Conformity

Data format

name of format

Comma-separated values (CSV)

version of format

unknown

Constraints related to access and use

Constraint set

Use constraints

Constraint set

responsible party role

publisher

Metadata on metadata

Metadata point of contact

organisation name

Environmental Information Data Centre

full postal address

Lancaster Environment Centre, Library Avenue, Bailrigg

Lancaster

LA1 4AP

email address

eidc@ceh.ac.uk

responsible party role

pointOfContact

Metadata date

2019-03-20T15:39:35

Metadata language

eng