Identification

Title

Dietary metabarcoding reads from stomachs of fish found in the Irish Sea, Celtic Sea, English Channel, and North Sea from 2015 to 2016

Abstract

Raw sequences produced in 2018 from the stomach samples of lemon sole (n=16), herring (n=36), whiting (n=27), plaice (n=24), haddock (n=15), saithe (n=20), horse mackerel (n=15), grey gurnard(n=15), and mackerel (n=38). Samples were obtained from the North Sea, Eastern channel, and Irish and Celtic Seas. The sequences were analysed using a MiSeq benchtop sequencer.

Resource type

dataset

Resource locator

https://data.cefas.co.uk/view/21645

name: Cefas Data Portal

description: The Cefas Data Portal contains metadata records and data sets available to download and connect to in support of our commitment to open science. Data is available in the following formats: Binary download.

function: download

Unique resource identifier

code

CEFAS21645

codeSpace

https://data.cefas.co.uk

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

biota

Keywords

Keyword set

keyword value

originating controlled vocabulary

title

GEMET, version 1.0

reference date

date type

publication

effective date

2008-06-01

Keyword set

keyword value

originating controlled vocabulary

title

GEMET - INSPIRE themes, version 1.0

reference date

date type

publication

effective date

2008-06-01

Keyword set

keyword value

originating controlled vocabulary

title

SeaVoX Vertical Co-ordinate Coverages

reference date

date type

revision

effective date

2010-05-18

Keyword set

keyword value

originating controlled vocabulary

title

MEDIN metadata record availability

reference date

date type

publication

effective date

2012-01-11

Keyword set

keyword value

originating controlled vocabulary

title

MEDIN metadata record availability

reference date

date type

publication

effective date

2012-01-11

Geographic location

West bounding longitude

-11.00

East bounding longitude

5.00

North bounding latitude

60.50

South bounding latitude

49.00

Temporal reference

Temporal extent

Begin position

2018-01

End position

2023-03-01

Dataset reference date

date type

publication

effective date

2023-03-01

date type

revision

effective date

2023-03-01

date type

creation

effective date

2023-02-24

Frequency of update

notPlanned

Quality and validity

Lineage

Full details are available in 5.3 of `*Resolving the role of jellyfish in marine food webs*`_. In brief: fish stomach samples were obtained from North Sea International Beam Trawl Survey (IBTS) (8 August – 6th September 2016), Eastern channel beam trawl (17th– 30th July 2016), and the Poseidon (3rd -21stOctober 2015) research cruises were collected aboard the RV Cefas Endeavour. Samples from the Celtic sea herring acoustic survey (CSHAS) (7th-27th October 2016) were collected aboard the RV Celtic explorer. Stomachs were removed and the contents DNA was extracted by salt for Poseidon samples, and CTAB DNA extraction for all other samples. 1st round PCRS were conducted using two sets of primers: ILF_ProSSU3 (18s) and Uni-Minibar (COI). Second round PCR attached unique nucleotide indexes. Samples were sequenced on MiSeq and demultiplexed at Sheffield diagnostic genetics services. .. _`*resolving the role of jellyfish in marine food webs*`: https://ueaeprints.uea.ac.uk/id/eprint/69971/1/2019LambPPhD.pdf

Conformity

Conformity report

specification

title

INSPIRE Data Specification on Species Distribution – Technical Guidelines

reference date

date type

publication

effective date

2013-12-10

degree

false

explanation

See the referenced specification

Conformity report

specification

title

reference date

date type

publication

effective date

2010-12-08

degree

true

explanation

See the referenced specification

Data format

name of format

Unknown

version of format

Constraints related to access and use

Constraint set

Limitations on public access

Constraint set

Limitations on public access

Responsible organisations

Responsible party

organisation name

Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS)

full postal address

Cefas Lowestoft Laboratory

Pakefield Road

Lowestoft

NR33 0HT

UK

email address

data.manager@cefas.co.uk

responsible party role

originator

Responsible party

organisation name

Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS)

full postal address

Cefas Lowestoft Laboratory

Pakefield Road

Lowestoft

NR33 0HT

UK

email address

data.manager@cefas.co.uk

responsible party role

custodian

Responsible party

organisation name

Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS)

full postal address

Cefas Lowestoft Laboratory

Pakefield Road

Lowestoft

NR33 0HT

UK

email address

data.manager@cefas.co.uk

responsible party role

distributor

Responsible party

organisation name

Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS)

email address

data.manager@cefas.co.uk

responsible party role

owner

Metadata on metadata

Metadata point of contact

organisation name

Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS)

full postal address

Cefas Lowestoft Laboratory

Pakefield Road

Lowestoft

NR33 0HT

UK

email address

data.manager@cefas.co.uk

responsible party role

pointOfContact

Metadata date

2023-03-01T04:14:34

Metadata language

eng