Identification

Title

Coastal Biodiversity and Ecosystem Service Sustainability (CBESS) surface sediment colloidal carbohydrate concentration in saltmarsh and mudflat habitats

Abstract

The dataset details surface sediment colloidal carbohydrate concentrations across six intertidal sites in the winter and summer of 2013. Colloidal carbohydrate concentrations in surface sediments (top 2mm) provide a quantitative measure of the extracellular polymeric substances (EPS) secreted by organisms that form the microphytobenthos (MPB) community. Three of the sites were in Morecambe Bay, North West England and three of the sites were in Essex, South East England, each of these sites consisted of a saltmarsh area and adjacent mudflat area, twenty two sampling quadrats were placed on each area. Five replicate sediment samples were taken at each quadrat and were analysed using the Dubois Phenol-Sulphuric Assay which involves spectrophotometer analysis of absorptions to determine colloidal carbohydrate concentrations. Values for colloidal carbohydrate concentrations are expressed as glucose equivalents in micrograms per gram of sediment. This data was collected as part of Coastal Biodiversity and Ecosystem Service Sustainability (CBESS): NE/J015644/1. The project was funded with support from the Biodiversity and Ecosystem Service Sustainability (BESS) programme. BESS is a six-year programme (2011-2017) funded by the UK Natural Environment Research Council (NERC) and the Biotechnology and Biological Sciences Research Council (BBSRC) as part of the UK's Living with Environmental Change (LWEC) programme. Full details about this dataset can be found at https://doi.org/10.5285/6c027bf9-b344-4178-b8b3-d78cf39f017f

Resource type

dataset

Resource locator

https://data-package.ceh.ac.uk/data/6c027bf9-b344-4178-b8b3-d78cf39f017f

description: Download a copy of this dataset

function: download

https://data-package.ceh.ac.uk/sd/6c027bf9-b344-4178-b8b3-d78cf39f017f.zip

description: Supporting information available to assist in re-use of this dataset

function: information

Unique resource identifier

code

https://catalogue.ceh.ac.uk/id/6c027bf9-b344-4178-b8b3-d78cf39f017f

codeSpace

doi:

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

oceans

Keywords

Keyword set

keyword value

Habitats and Biotopes

originating controlled vocabulary

title

GEMET - INSPIRE themes, version 1.0

reference date

date type

publication

effective date

2008-06-01

Geographic location

West bounding longitude

-3

East bounding longitude

1.005

North bounding latitude

54.25

South bounding latitude

51.687

Temporal reference

Temporal extent

Begin position

2013-01-01

End position

2013-08-31

Dataset reference date

date type

publication

effective date

2016-04-25

date type

creation

effective date

2013-08-31

Frequency of update

notPlanned

Quality and validity

Lineage

Sediment colloidal carbohydrate concentration was determined through the collection of contact cores. Contact cores use liquid nitrogen to freeze the top 2mm of the sediment surface, excess unfrozen sediment is removed and the sample core is stored below -80 degree centigrade. The sample is weighed to determine wet weight and freeze dried to remove all water. Colloidal carbohydrate concentration is determined using the Dubois Phenol-Sulphuric assay. A sediment sub-sample (50-500mg, depending on site) is taken from dried contact core sediment and known volume of distilled water is added. Sample is vortexed and centrifuged then a set volume of supernatant is removed. Concentrated sulphuric acid and 5 percent phenol are added to the supernatant sample ensuring the ratio of sample: phenol: acid is kept at 1:1:5 by volume. Allow 35mins for colour to develop. Decant the samples into cuvettes and read the absorbance at 486.5nm on Thermo Biomate 5 spectrophotometer. Use figures from regression analysis of glucose standards to calculate colloidal carbohydrate concentration expressed as glucose equivalents in micrograms per gram (µg g-1). Note: zeroes indicate colloidal carbohydrate concentration below detectable level. Twenty two 1 x 1 m quadrats were randomly allocated to each mudflat and saltmarsh site using R (R Development Core Team, 2014) to specify four different spatial scales (A = 1 quadrat only, B = 3 quadrats at 1 m to 10 m apart, C = 6 quadrats at 10 m to 100 m apart, D = 12 quadrats at 100 m to 1000 m or site maximum).

Conformity

Conformity report

specification

title

Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services

reference date

date type

publication

effective date

2010-12-08

degree

explanation

Data format

name of format

Comma-separated values (CSV)

version of format

Constraints related to access and use

Constraint set

Limitations on public access

Constraint set

Limitations on public access

Constraint set

Limitations on public access

Constraint set

Limitations on public access

If you reuse this data, you should cite: Maunder, J., Paterson, D.M. (2016). Coastal Biodiversity and Ecosystem Service Sustainability (CBESS) surface sediment colloidal carbohydrate concentration in saltmarsh and mudflat habitats. NERC Environmental Information Data Centre https://doi.org/10.5285/6c027bf9-b344-4178-b8b3-d78cf39f017f

Responsible organisations

Metadata on metadata

Metadata point of contact

organisation name

full postal address

Lancaster Environment Centre, Library Avenue, Bailrigg

Lancaster

LA1 4AP

email address

info@eidc.ac.uk

web address

https://eidc.ac.uk/

description: The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences.

function: information

responsible party role

pointOfContact

Metadata date

2024-02-27T16:28:30

Metadata language

eng