Identification

Title

2010 - 2019 Centre for Environment, Fisheries & Aquaculture Science (Cefas) Northwest European shelf seas - Phytoplankton pigments - 2010 to 2019

Abstract

These data contain concentrations in micrograms per litre (ug/L) of the individual accessory pigments of phytoplankton measured by High Pressure Liquid Chromatography (HPLC). Pigment samples were taken primarily on Cefas fisheries and SmartBuoy cruises and sent to DHI laboratory in Denmark for HPLC analysis. Data was then sent back to Cefas and matched with metadata and quality controlled. Chlorophyll concentrations from this dataset have a variety of uses: for assessment of concentrations for purposes such as eutrophication assessments, validation of remote sensing derived chlorophyll estimates, as a proxy for phytoplankton biomass, or to calibrate fluorometers. Pigment concentrations can be applied like a fingerprint to derive phytoplankton functional types and give information about the phytoplankton community.

Resource type

dataset

Resource locator

http://data.cefas.co.uk/#/View/53/

function: order

Unique resource identifier

code

CEFAS5db0317a-6080-44d8-8c65-0cad0853c646

codeSpace

http://www.cefas.co.uk/

Dataset language

eng

Spatial reference system

authority code

OGP

code identifying the spatial reference system

urn:ogc:def:crs:EPSG::4326

Classification of spatial data and services

Topic category

biota

Keywords

Keyword set

keyword value

Keyword set

keyword value

originating controlled vocabulary

title

reference date

date type

revision

effective date

2011-03-25

Keyword set

keyword value

originating controlled vocabulary

title

reference date

date type

publication

effective date

2008-06-01

Keyword set

keyword value

originating controlled vocabulary

title

reference date

date type

publication

effective date

2008-06-01

Geographic location

West bounding longitude

-13

East bounding longitude

14

North bounding latitude

62.5

South bounding latitude

47.5

Extent

Extent group

authority code

title

reference date

date type

revision

effective date

2010-05-18

code identifying the extent

Extent group

authority code

title

reference date

date type

revision

effective date

2010-05-18

code identifying the extent

Temporal reference

Temporal extent

Begin position

2010-01-08

End position

2019-10-26T23:00:00.000Z

Dataset reference date

date type

publication

effective date

2020-11-11

Frequency of update

quarterly

Quality and validity

Lineage

2 litre water samples were collected on board the RV Cefas Endeavour from the continuous flow ferrybox water supply (4m depth), or from Niskin bottles on a rosette fired at particular depths. Water samples were filtered through a Whatman GFF filter, and immediately frozen in liquid nitrogen (2010-2012) or in a -80°C freezer on board (other years). Pigments were stored at -80°C for 1-4 months, before being selected for analysis, then transferred to the analysis laboratory (DHI; Horsholm, Denmark) using dry ice. Pigments were extracted adding 6 ml of 95% acetone (and an internal standard, vitamin E) to each filter. The samples were sonicated in an ice-cold sonication bath for 10 min and extracted at 4 °C for 20 hours. The samples were then filtered through 0.2 µm Teflon syringe filter into HPLC vials. Buffer and samples were injected on HPLC (Shimadzu LC-10A HPLC system with LC Solution software) in the ratio 5:2 using a pre-treatment program and mixing in the loop before injection (Schlüter et al 2016). The HPLC method used was the Van Heukelem & Thomas (2005) method where the gradient was adjusted to optimise the resolution of the pigments. The raw data were stored in an MS Access database. Quality control was performed after Aiken et al 2009. Samples were removed if they had insufficient chlorophyll (<0.04ug/L), a ratio of above 0.5 of degradation pigments (chlorophyllide + pheophorbide + pheophytin) to chlorophyll a, or if there was a difference between total chlorophyll a (TChla) and accessory pigments (AP) of more than 40% of the total pigments. Additionally, where at least 5 samples were collected for a survey, total chlorophyll a was regressed against total accessory pigments, and the data from the survey was rejected if the regression was poor (r2<0.75, slope <0.65 or <1.4).

Conformity

Data format

name of format

version of format

Constraints related to access and use

Constraint set

Use constraints

Contact the Cefas Data Manager for more information

Constraint set

Limitations on public access

Contact the Cefas Data Manager for more information

Responsible organisations

Responsible party

contact position

Data Manager

organisation name

Centre for Environment, Fisheries & Aquaculture Science

full postal address

Cefas Lowestoft Laboratory

Pakefield Road

Lowestoft

NR33 0HT

UK

telephone number

+44 (0)1502 562244

email address

data.manager@cefas.co.uk

responsible party role

originator

Responsible party

contact position

Data Manager

organisation name

Centre for Environment, Fisheries & Aquaculture Science

full postal address

Cefas Lowestoft Laboratory

Pakefield Road

Lowestoft

NR33 0HT

UK

telephone number

+44 (0)1502 562244

email address

data.manager@cefas.co.uk

responsible party role

custodian

Metadata on metadata

Metadata point of contact

contact position

Data Manager

organisation name

Centre for Environment, Fisheries & Aquaculture Science

full postal address

Cefas Lowestoft Laboratory

Pakefield Road

Lowestoft

NR33 0HT

UK

telephone number

+44 (0)1502 562244

email address

data.manager@cefas.co.uk

responsible party role

pointOfContact

Metadata date

2020-11-11T11:25:13

Metadata language