Identification

Title

Fine root phosphatase enzyme activity in fertilised plots in Central Amazon, 2018

Abstract

The data consists of potential phosphatase activity released by fine roots in old growth forests in Central Amazon. Fine roots younger than three months were sampled using the ingrowth core technique in a large-scale nutrient fertilisation experiment. Phosphatase activity is given as a mean of the plant community per plot, where five points inside each plot were sampled and separated in two different soil layers (0-10 and 10-30cm). Samples were collected in February 2018, eight months after the nutrient fertilisation started at the AFEX project area in Manaus, Brazil at the Biological Dynamics of Forest Fragments Project (BDFFP/ INPA). The study was funded by NERC, BDFFP (logistical support) and Brazilian government (student scholarship) Full details about this dataset can be found at https://doi.org/10.5285/6529004d-b6e8-429d-ac68-0f5324d7e5d0

Resource type

dataset

Resource locator

https://data-package.ceh.ac.uk/data/6529004d-b6e8-429d-ac68-0f5324d7e5d0

description: Download a copy of this data

function: download

https://data-package.ceh.ac.uk/sd/6529004d-b6e8-429d-ac68-0f5324d7e5d0.zip

description: Supporting information available to assist in re-use of this dataset

function: information

Unique resource identifier

code

https://catalogue.ceh.ac.uk/id/6529004d-b6e8-429d-ac68-0f5324d7e5d0

codeSpace

doi:

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

biota

environment

Keywords

Geographic location

West bounding longitude

-68.5

East bounding longitude

-31.7

North bounding latitude

5.1

South bounding latitude

-24.6

Temporal reference

Temporal extent

Begin position

2018-02-01

End position

2018-09-30

Dataset reference date

date type

publication

effective date

2020-04-03

Frequency of update

Quality and validity

Lineage

Root tips, that usually to refer absorptive fine roots directly responsible for nutrient acquisition, were cut from root sampled using ingrowth cores and potential root acid phosphomonoesterase activity was measured within 3 days of root sampling using a fluorimetric microplate assay. Roots were incubated with Methylumbelliferyl-phosphate (MUF) for 30 minutes at ~ 25 ˚C while gently shaking, and after the termination of the reaction fluorescence was read on a fluorometer (Tecan Infinite® 200 PRO, Grödig, Austria), at 365 nm excitation and 450 nm emission. Root phosphatase activity per plot was expressed in μmol MUF g−1 dry mass h

Conformity

Conformity report

specification

title

Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services

reference date

date type

publication

effective date

2010-12-08

degree

explanation

Data format

name of format

Comma-separated values (CSV)

version of format

Constraints related to access and use

Constraint set

Limitations on public access

Constraint set

Limitations on public access

Natural Environment Research Council

Constraint set

Limitations on public access

Constraint set

Limitations on public access

If you reuse this data, you should cite: Lugli, L.F., Quesada, C.A.N., Andersen, K.M., Mercado, L.M., Fuchslueger, L., Martins, N.P., Schaap, K.J., Hartley, I.P. (2020). Fine root phosphatase enzyme activity in fertilised plots in Central Amazon, 2018. NERC Environmental Information Data Centre https://doi.org/10.5285/6529004d-b6e8-429d-ac68-0f5324d7e5d0

Responsible organisations

Responsible party

organisation name

email address

laynaralugli@gmail.com

web address

https://orcid.org/0000-0001-8404-4841

description: ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers.

function: information