Telomere length data from a wild population of Seychelles Warblers on Cousin Island, 1995-2014
These data consist of relative telomere length (RTL) measures from quantitative polymerase chain reaction, of Seychelles warbler birds on Cousin Island, Seychelles. The data were collected by the Seychelles Warbler Project in 1995-2014. Data include bird identity, sex, age, birth period, qPCR plate identity, RTL, technician, territory, field period, mum ID, dad ID, mum age at conception, dad age at conception, dominant female ID in the natal territory, dominant male ID in the natal territory Full details about this dataset can be found at https://doi.org/10.5285/8a8240a2-e8ed-495d-ae93-c35200956764
dataset
https://data-package.ceh.ac.uk/data/8a8240a2-e8ed-495d-ae93-c35200956764
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https://data-package.ceh.ac.uk/sd/8a8240a2-e8ed-495d-ae93-c35200956764.zip
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description: Supporting information available to assist in re-use of this dataset
function: information
https://catalogue.ceh.ac.uk/id/8a8240a2-e8ed-495d-ae93-c35200956764
doi:
eng
biota
55.645
55.679
-4.316
-4.345
1995-01-01
2014-12-31
publication
2021-07-26
creation
2021-06-24
Blood samples (~25 μl) were collected and stored at room temperature in absolute ethanol. DNA was extracted using a DNeasy blood and tissue kit (Qiagen) according to the manufacturer’s instructions with modification of overnight lysis at 37 °C and a final DNA elution volume of 80 μl. DNA concentration and purity were quantified using a NanoDrop 8000 Spectrophotometer (ThermoScientific). The following thresholds were applied before samples were included for further analysis: (1) DNA concentration must be at least 15 ng/μl (based on a mean of three measurements), (2) the 260/280 absorbance ratio has to be between 1.8 and 2.0 for acceptable DNA purity, and (3) the 260/230 absorbance ratio must be higher than 1.8. DNA integrity was further validated by visualisation with ethidium bromide after electrophoresis on a 1.2% agarose gel, and all samples with evidence of DNA degradation were re-extracted or excluded. All DNA extractions that passed the above criteria were diluted to 3.3 ng/μl before telomere measurement. We used quantitative polymerase chain reaction to calculate a relative measure of telomere length that describes the amount of telomeric DNA in a sample relative to that of GAPDH, a constantly expressed reference gene.
publication
2010-12-08
Comma-separated values (CSV)
Controlled
If you reuse this data, you should cite: Sparks, A.M., Spurgin, L.G., van der Velde, M., Fairfield, E.A., Komdeur, J., Burke, T., Richardson, D.S., Dugdale, H.L. (2021). Telomere length data from a wild population of Seychelles Warblers on Cousin Island, 1995-2014. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/8a8240a2-e8ed-495d-ae93-c35200956764
University of Leeds
https://orcid.org/0000-0002-7697-4632
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author
University of East Anglia
https://orcid.org/0000-0002-0874-9281
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author
University of Groningen
author
University of East Anglia
author
University of Groningen
author
University of Sheffield
https://orcid.org/0000-0003-3848-1244
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description: ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers.
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University of East Anglia
https://orcid.org/0000-0001-7226-9074
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University of Groningen
https://orcid.org/0000-0001-8769-0099
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description: ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers.
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University of Groningen
https://orcid.org/0000-0001-8769-0099
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University of Leeds
https://orcid.org/0000-0001-8769-0099
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owner
Lancaster Environment Centre, Library Avenue, Bailrigg
Lancaster
LA1 4AP
UK
name: EIDC website
description: The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences.
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2024-03-05T08:54:24