Identification

Title

Bacteroides phages recovered from human and animal faecal matrices in rural Kenya

Abstract

This data describes the recovering and isolation processes of Bacteroides spp. strains from human and cattle faecal sources from rural areas in Siaya County (Kenya), and occurred between 7th and 28th of June 2018. The data also includes the detection of bacteriophages (infecting these Bacteroides spp. host strains) in conjunction with traditional faecal indicator organisms in water sources from Kisumu and Siaya County (Kenya) occurring between June 18th 2018 and June 13th 2019. Exact location (coordinates) of the sample points are also described in the data set. A microbiological technique using Bile Esculin Bacteroides (BBE) agar was used for the recovering and isolation processes of Bacteroides spp. strains. Standard ISO (7899-2, 9308-1, 10705-2 and 10705-4) techniques, such as membrane filtration and the double-agar-layer methods, were used for the detection of bacteriophages and traditional faecal indicator organisms. The purpose of data collection was to develop new markers that could identify cattle and/or human sources of faecal contamination, which could be used as part of a Microbial Source Tracking (MST) tool box. Technicians and researchers from the University of Brighton (UK), University of Southampton (UK), from the Victoria Institute for Research on Environment and Development (VIRED) (KE) and from the Kenya Medical Research Institute (KEMRI) (KE) were responsible for the collection and interpretation of data. Full details about this dataset can be found at https://doi.org/10.5285/02c8a6b0-e59e-4278-b9a2-9958cd5a2c3c

Resource type

dataset

Resource locator

https://data-package.ceh.ac.uk/data/02c8a6b0-e59e-4278-b9a2-9958cd5a2c3c

description: Download a copy of this data

function: download

https://data-package.ceh.ac.uk/sd/02c8a6b0-e59e-4278-b9a2-9958cd5a2c3c.zip

description: Supporting information available to assist in re-use of this dataset

function: information

Unique resource identifier

code

https://catalogue.ceh.ac.uk/id/02c8a6b0-e59e-4278-b9a2-9958cd5a2c3c

codeSpace

doi:

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

environment

Keywords

Geographic location

West bounding longitude

33.267

East bounding longitude

42.54

North bounding latitude

4.873

South bounding latitude

-4.525

Temporal reference

Temporal extent

Begin position

2018-06-01

End position

2019-06-30

Dataset reference date

date type

publication

effective date

2019-12-06

Frequency of update

Quality and validity

Lineage

The data came into existence after three steps: firstly a field collection of cattle and human faecal material for the isolation and recovering of Bacteroides spp. host strains ; secondly laboratory work were performed for the isolation of those strains; and thirdly field and laboratory work were done for assessing levels of bacteriophages and faecal indicator organism in environmental waters. Besides basic PPE (e.g.: gloves, glasses,..), fieldwork instrumentation included sterile wooden spatulas and an adapted sampling pole for the collection of cattle and human faeces from water pan shorelines and school pit latrines, respectively. Faecal and water samples were contained in sterile plastic containers in cooler bags containing ice packs and transported to KEMRI Kisian laboratory within 4 hours of collection. In KEMRI Kisian laboratory a total of 10 (5 from cattle and 5 from human faeces) Bacteroides spp. strains were recovered, isolated and preserved (frozen) for posterior use. These recently isolated Bacteroides spp. were then used as hosts, in the double-agar-layer method, for the detection of bacteriophages in Kenyan water sources. The units used for the detection of bacteriophages and traditional faecal indicator organism were plaque and /or colony forming units per 1 mL or 100 mL of water sample (PFU / 1 mL; PFU / 100 mL and CFU / 100 mL). Calibration of laboratory instrumentation as well as the use of blank samples (distilled water) were employed as data quality controls.

Conformity

Conformity report

specification

title

Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services

reference date

date type

publication

effective date

2010-12-08

degree

explanation

Data format

name of format

Comma-separated values (CSV)

version of format

Constraints related to access and use

Constraint set

Limitations on public access

Constraint set

Limitations on public access

Constraint set

Limitations on public access

If you reuse this data, you should cite: Trajano Gomes da Silva, D., Ebdon, J. , Mwangi, T., Okotto-okotto, J., Wright, J. (2019). Bacteroides phages recovered from human and animal faecal matrices in rural Kenya. NERC Environmental Information Data Centre https://doi.org/10.5285/02c8a6b0-e59e-4278-b9a2-9958cd5a2c3c

Responsible organisations

Responsible party

organisation name

University of Brighton

email address

D.TrajanoGomesdaSilva2@brighton.ac.uk

web address

https://orcid.org/0000-0003-1971-2799

description: ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers.

function: information