Microbial DNA extraction and PCR from core samples SSK105556 and SSK105560 from UKGEOS Glasgow Observatory, borehole GGC01
Microbial DNA concentrations from DNA extraction using Qiagen PowerSoil Pro DNA Extraction kit and subsequent Polymerase Chain Reaction (PCR) from core samples collected and preserved for microbiology (SSK105556 and SSK105560) from UKGEOS Glasgow Observatory, borehole GGC01. DNA extractions and subsequent amplification using PCR indicate levels of microbial DNA below detection limits. NERC grant SHAPE-UK NE/R018006/1.
dataset
https://webapps.bgs.ac.uk/services/ngdc/accessions/index.html#item181843
name: Data
function: download
http://data.bgs.ac.uk/id/dataHolding/13608095
eng
geoscientificInformation
publication
2008-06-01
Abrasion
Geomicrobiology
NGDC Deposited Data
Scottish SDI
Sandstone
Shale
revision
2022
NERC_DDC
-4.2200
-4.2200
55.8400
55.8400
revision
2002
Glasgow [id=1298677]
2021-06-01
2021-06-30
creation
2023-08-03
notApplicable
Detailed methodology in attached documents. Core samples processed into powder in sterile conditions. Powdered core then used for DNA extractions in Qiagen PowerSoil Pro DNA Extraction kit. Resulting elutes amplified using Polymerase Chain Reaction (PCR). PCR products visualised using gel electrophoresis.
publication
2011
false
See the referenced specification
publication
2010-12-08
false
See http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2010:323:0011:0102:EN:PDF
MS Excel
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