Identification

Title

Population dynamics of evolving bacterial populations in liquid media, 2020

Abstract

This data was collected from co-evolving bacterial populations containing Pseudomonas fluorescens strain SBW25 and a plasmid, pQBR57. The composition of the community was tracked using flow cytometry to distinguish 1) an unlabelled wild type strain 2) a dTomato compensated host (SBW25 KO PFLU4242), and 3) a wild type host bearing a Green fluorescent protein (GFP) labelled compensated plasmid (pQBR57 KO 0059). Full details about this dataset can be found at https://doi.org/10.5285/51046841-deaa-422f-a303-2c0759f014b4

Resource type

dataset

Resource locator

https://data-package.ceh.ac.uk/data/51046841-deaa-422f-a303-2c0759f014b4

name: Download the data

description: Download a copy of this data

function: download

https://data-package.ceh.ac.uk/sd/51046841-deaa-422f-a303-2c0759f014b4.zip

name: Supporting information

description: Supporting information available to assist in re-use of this dataset

function: information

Unique resource identifier

code

https://catalogue.ceh.ac.uk/id/51046841-deaa-422f-a303-2c0759f014b4

codeSpace

doi:

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Classification of spatial data and services

Topic category

biota

Keywords

Keyword set

keyword value

Environmental Monitoring Facilities

originating controlled vocabulary

title

reference date

date type

publication

effective date

2008-06-01

Keyword set

keyword value

co-evolution

plasmid compensation

SBW25

pQBR57

Geographic location

West bounding longitude

-8.648

East bounding longitude

1.768

North bounding latitude

60.861

South bounding latitude

49.864

Temporal reference

Temporal extent

Begin position

2020-01-01

End position

2020-12-31

Dataset reference date

date type

publication

effective date

2022-11-23

Frequency of update

Quality and validity

Lineage

Flow cytometry data was filtered to remove background noise (using thresholds in forward scatter (FSC-H), side scatter (SSC-H) and blue signal (PB450-H) channels), then all events were classified by fluorescence (flow_data.txt) to provide a community composition for each sample recorded as population fractions (fraction_data.txt).

Conformity

Conformity report

specification

title

reference date

date type

publication

effective date

2010-12-08

degree

explanation

Data format

name of format

text

version of format

Constraints related to access and use

Constraint set

Limitations on public access

Constraint set

Limitations on public access

Constraint set

Limitations on public access

If you reuse this data, you should cite: Wright, R. (2022). Population dynamics of evolving bacterial populations in liquid media, 2020. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/51046841-deaa-422f-a303-2c0759f014b4

Responsible organisations

Responsible party

organisation name

University of Manchester

email address

enquiries@ceh.ac.uk

responsible party role

author

Responsible party

organisation name

University of Manchester

email address

enquiries@ceh.ac.uk

responsible party role

pointOfContact

Responsible party

organisation name

NERC EDS Environmental Information Data Centre

email address

enquiries@ceh.ac.uk

responsible party role

custodian

Responsible party

organisation name

NERC EDS Environmental Information Data Centre

email address

enquiries@ceh.ac.uk

responsible party role

publisher

Responsible party

organisation name

University of Manchester

email address

enquiries@ceh.ac.uk

responsible party role

owner

Metadata on metadata

Metadata point of contact

organisation name

Environmental Information Data Centre

full postal address

Lancaster Environment Centre, Library Avenue, Bailrigg

Lancaster

LA1 4AP

UK

email address

info@eidc.ac.uk

responsible party role

pointOfContact

Metadata date

2022-11-25T11:18:32

Metadata language